Anti-SPRR3 antibody (ab218131)
Key features and details
- Rabbit polyclonal to SPRR3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPRR3 antibody -
Description
Rabbit polyclonal to SPRR3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SPRR3 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UBC9 -
Positive control
- Human laryngocarcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cross-linked envelope protein of keratinocytes. -
Sequence similarities
Belongs to the cornifin (SPRR) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6707 Human
- Entrez Gene: 20766 Mouse
- Entrez Gene: 310575 Rat
- Omim: 182271 Human
- SwissProt: Q9UBC9 Human
- SwissProt: O09116 Mouse
- SwissProt: D3ZHQ6 Rat
- Unigene: 139322 Human
see all -
Alternative names
- 22 kDa pancornulin antibody
- Cornifin beta antibody
- Esophagin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRR3 antibody (ab218131)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human laryngocarcinoma tissue, labeling SPRR3 using ab218131 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab218131 has been referenced in 1 publication.
- Yu L et al. Identification of SPRR3 as a novel diagnostic/prognostic biomarker for oral squamous cell carcinoma via RNA sequencing and bioinformatic analyses. PeerJ 8:e9393 (2020). PubMed: 32596058