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Validated using a knockout cell line

Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)

Reviews (36)Q&A (4)References (918)
Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Immunoprecipitation - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
  • Flow Cytometry - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)

Key features and details

  • Mouse monoclonal [2C11] to SQSTM1 / p62 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free
    See all SQSTM1 / p62 primary antibodies

  • Description

    Mouse monoclonal [2C11] to SQSTM1 / p62 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IP, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human SQSTM1/ p62 aa 1-440.
    Database link: Q13501

  • Positive control

    • WB: HeLa, Hap1, U-2 OS cell lysates. Flow Cyt: HeLa. IHC-P: Human lymph node. ICC/IF: HeLa and U-2 OS cells. IP: U-2 OS cell lysate

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab56416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (3)
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP (1)
Use at an assay dependent concentration.
WB (23)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.

Fix cells for 15 minutes with 2 mL of 4% paraformaldehyde solution (pH 7.4 with NaOH in PBS). Permeabilize cells by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody
Notes
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Fix cells for 15 minutes with 2 mL of 4% paraformaldehyde solution (pH 7.4 with NaOH in PBS). Permeabilize cells by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody

Target

  • Function

    Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

  • Tissue specificity

    Ubiquitously expressed.

  • Involvement in disease

    Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

  • Sequence similarities

    Contains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.

  • Domain

    The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
    The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
    The ZZ-type zinc finger mediates the interaction with RIPK1.

  • Post-translational
    modifications

    Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

  • Cellular localization

    Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
  • Information by UniProt

  • Database links

    • Alternative names

      • A170 antibody
      • DMRV antibody
      • EBI 3 associated protein of 60 kDa antibody
      • EBI 3 associated protein p60 antibody
      • EBI3 associated protein of 60 kDa antibody
      • EBI3 associated protein p60 antibody
      • EBI3-associated protein of 60 kDa antibody
      • EBIAP antibody
      • FTDALS3 antibody
      • MGC127197 antibody
      • ORCA antibody
      • OSF-6 antibody
      • Osi antibody
      • OSIL antibody
      • Oxidative stress induced like antibody
      • p60 antibody
      • p62 antibody
      • p62B antibody
      • Paget disease of bone 3 antibody
      • PDB 3 antibody
      • PDB3 antibody
      • Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
      • Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
      • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
      • PKC-zeta-interacting protein antibody
      • Protein kinase C-zeta-interacting protein antibody
      • Sequestosome 1 antibody
      • Sequestosome-1 antibody
      • SQSTM 1 antibody
      • SQSTM_HUMAN antibody
      • Sqstm1 antibody
      • STAP antibody
      • STONE14 antibody
      • Ubiquitin binding protein p62 antibody
      • Ubiquitin-binding protein p62 antibody
      • ZIP 3 antibody
      • ZIP antibody
      • ZIP3 antibody
      see all

    Images

    • Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      All lanes : Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : SQSTM1 knockout HEK293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Observed band size: 62 kDa why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab56416 observed at 62 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

       ab56416 was shown to react with SQSTM1/p62 in wild-type HEK293T cells in western blot. Loss of signal was observed when knockout cell line ab255343 (knockout cell lysate ab263770) was used. Wild-type HEK293T and SQSTM1 knockout HEK293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab56416 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      ab56416 was shown to react with SQSTM1 in wild-type U-2 OS cells in Immunocytochemistry with loss of signal observed in a SQSTM1 knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1/10 000. The cells were then incubated with ab56416 at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-mouse secondary antibody to (Alexa Fluor® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880). These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.ing knock out cell lines.
    • Immunoprecipitation - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Immunoprecipitation - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Immunoprecipitation of SQSTM1 in U-2 OS cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab56416 pre-coupled to prot.G-Sepharose beads. Samples were washed and processed for western blot with ab207305 at 1/10,000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
    • Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      All lanes : Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416) at 1/1000 dilution

      Lane 1 : Wild-type U-2 OS cell lysate
      Lane 2 : SQSTM1 knockout U-2 OS cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.


      ab56416 was shown to react with SQSTM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a SQSTM1 knockout cell line. Wild-type U-2 OS and SQSTM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab56416 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-mouse HRP secondary antibodies at 0.3ug/mL before imaging. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.

    • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)

      Monoclonal antibody to SQSTM1 (ab56416) on HeLa cell, antibody concentration 10 ug/ml.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)

      ab56416 (1µg/ml) staining SQSTM1 in human lymph node using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)
      Flow Cytometry - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (ab56416)

      Overlay histogram showing HeLa cells stained with ab56416 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56416, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    References (918)

    Publishing research using ab56416? Please let us know so that we can cite the reference in this datasheet.

    ab56416 has been referenced in 918 publications.

    • Stritt S  et al. APOLD1 loss causes endothelial dysfunction involving cell junctions, cytoskeletal architecture, and Weibel-Palade bodies, while disrupting hemostasis. Haematologica 108:772-784 (2023). PubMed: 35638551
    • Lumkwana D  et al. Investigating the Role of Spermidine in a Model System of Alzheimer's Disease Using Correlative Microscopy and Super-resolution Techniques. Front Cell Dev Biol 10:819571 (2022). PubMed: 35656544
    • Abdel-Moety A  et al. SQSTM1 Expression in Hepatocellular Carcinoma and Relation to Tumor Recurrence After Radiofrequency Ablation. J Clin Exp Hepatol 12:774-784 (2022). PubMed: 35677515
    • Triolo M  et al. The influence of age, sex, and exercise on autophagy, mitophagy, and lysosome biogenesis in skeletal muscle. Skelet Muscle 12:13 (2022). PubMed: 35690879
    • Zeng YH  et al. GGC Repeat Expansion of RILPL1 is Associated with Oculopharyngodistal Myopathy. Ann Neurol 92:512-526 (2022). PubMed: 35700120
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    31-40 of 40 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Skeletal Muscle)
    Permeabilization
    No
    Specification
    Skeletal Muscle
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Methacarn
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 01 2013

    Western blot abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    40 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Randal Tibbetts

    Verified customer

    Submitted Jan 24 2012

    Western blot abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Primary Breast Cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    200 µg
    Specification
    Primary Breast Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 06 2010

    Western blot abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1 cells)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    THP-1 cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 29 2010

    Western blot abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat cardiomyoctye and H9C2 cell line rat myocyte l)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    rat cardiomyoctye and H9C2 cell line rat myocyte l
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Prof. Paul Townsend

    Verified customer

    Submitted Mar 17 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SQSTM1 / p62 antibody - Autophagosome Marker

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A431 epidermoid cancer cells)
    Permeabilization
    Yes - triton x-100
    Specification
    A431 epidermoid cancer cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alejandro Vazquez-Martin

    Verified customer

    Submitted Dec 02 2009

    Question

    Thank you for reply. I agree that none of these ABs show concentration in the data sheet, and the agarose conjugate in p62 Ab will affect the Cy5-labling. However, I think the difference in the concentration will only affect the labeling efficiency, not the specificity of the antibody. Even if there are very high back ground caused by free conjugates, I supposed to be able to see more staining in the cell body, not the opposite (like what I got). I tried once again (with/without antigen retrieval) using ONLY the Cy5-conjugated antibodies, and the results are the same.
    I'll try FITC labeling with the AbCam p62 AB (CAT#ab56416). Any suggestion? Can I add more quench buffer to eliminate the background as mush as possible? Thank you!

    Read More
    Answer

    Thank you for your reply.

    Yes, the ab56416 should be very compatible with the EasyLink kits. The concentration is 1 mg/ml, it's protein G purified, and it has no preservatives/carriers in the buffer.

    You could try to add more quencher. Theoretically, the 1 µl of EL-Quencher FD reagent for every 10 µl of antibody should be more than enough, but if you try more it shouldn't have any adverse effect.

    Please let me know that the results are for the FITC-labeled ab56416. This combination should work well. I look forward to your reply.

    Read More

    Question

    Dear all

    We have a Neuropathology customer that wants to know the best clone/part number from the following ABCAM antidobies:

    p62/SQSTM1

    anti-isocitrate dehydrogenase antibody
    Please be aware that both are for IHC in Neuropathology
    --

    Read More
    Answer

    Thank you for contacting us.

    As an antibody for p62/SQSTM1 I can recommend the ab56416. This is a monoclonal antibody which is tested in paraffin embedded IHC and on human tissue. It has been used in many publication as well. Please see here a link to the datasheet: https://www.abcam.com/SQSTM1--p62-antibody-Autophagosome-Marker-ab56416.html (or use the following: https://www.abcam.com/SQSTM1--p62-antibody-Autophagosome-Marker-ab56416.html).

    As an antibody for isocitrate dehydrogenase I can recommend the ab117976, which is also a monoclonal antibody tested in IHC and on human tissue. Please see here a link to the datasheet: https://www.abcam.com/IDH1-antibody-2H9-ab117976.html (or use the following: https://www.abcam.com/IDH1-antibody-2H9-ab117976.html).

    I would just also like to remind you, that our antibodies are for research use and not diagnostics.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I was wondering what is your guarantee for the antibodies.

    Read More
    Answer

    The guarantee is a promise of a replacement, credit, or refund if an antibody fails in an application listed on the datasheet, with samples derived from the species listed in the "Cross-reactivity" field of the datasheet. For ab2787, as an example, the guarantee applies to:

    Application: ICC, IHC-P, IP, WB

    Species: Human, Mouse, Rat, Cow, Fruit fly (Drosophila melanogaster), Rabbit, Saccharomyces cerevisiae and Xenopus laevis

    The species listed as "predicted" are not covered by the guarantee. The guarantee is in effect for 6 months following purchase.

    The following link has a fuller description:

    www.abcam.com/abpromise

    If you have any questions, please contact me.

    Read More

    Question

    Goodmorning,

    I want to use antibody against p62/Sequestome-1 in immunoprecipitation assays. I want the antibody to be free of glycerol and sodium azide. I found one antibody that in fits to my conditions but you don’t specify if it works in immunoprecipitation assays. Also in the publication list it’s not been tested for immunoprecipitation. Can you provide me this information? Is this antibody applicable for IPs? Have the company tested or not? Or you can send as an aliquot to check it if you haven’t done it yet.

    Thank you in advance.

    I am waiting for you reply as soon as possible.

    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately ab56416 has never been tested in IP assays. It doesn’t mean it won’t work in this application, but we don’t have data to support it.

    If you are interested in participating in our antibodies evaluation system (Abreview), and test ab56416 by Immunoprecipitation I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

    Therefore, I can offer a discount off a future purchase if you buy the antibody and test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab56416 in immunoprecipitation. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

    3. Test it in immunoprecipitation with one of the species stated on the datasheet.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: http://it.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    31-40 of 40 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com