Anti-SQSTM1 / p62 antibody (ab91526)
Key features and details
- Rabbit polyclonal to SQSTM1 / p62
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SQSTM1 / p62 antibody
See all SQSTM1 / p62 primary antibodies -
Description
Rabbit polyclonal to SQSTM1 / p62 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human SQSTM1/ p62 (C terminal).
Database link: Q13501 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).
Only mouse and human species are validated in WB. Rat is not a validated species. |
| IHC-P |
Use a concentration of 2 - 5 µg/ml.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa). Only mouse and human species are validated in WB. Rat is not a validated species. |
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IHC-P
Use a concentration of 2 - 5 µg/ml. |
Target
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Function
Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years. -
Sequence similarities
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
Domain
The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1. -
Post-translational
modificationsPhosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. -
Cellular localization
Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. - Information by UniProt
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Database links
- Entrez Gene: 8878 Human
- Entrez Gene: 18412 Mouse
- Entrez Gene: 113894 Rat
- Omim: 601530 Human
- SwissProt: Q13501 Human
- SwissProt: Q64337 Mouse
- SwissProt: O08623 Rat
- Unigene: 709030 Human
see all -
Alternative names
- A170 antibody
- DMRV antibody
- EBI 3 associated protein of 60 kDa antibody
see all
Images
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Western blot - Anti-SQSTM1 / p62 antibody (ab91526)All lanes : Anti-SQSTM1 / p62 antibody (ab91526) at 0.5 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 13 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 14 : L1210 (mouse lymphocytic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)Han, Z. et al PLoS One. 2014 Jan 23;9(1):e86838. doi: 10.1371/journal.pone.0086838. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling SQSTM1 / p62 with ab91526. Heat mediated antigen retrieval was performed. The tissue sections were then blocked with 10% goat serum in PBS, and incubated with primary antibody overnight at 4°C. Sections were incubated with secondary antibody for 1 h at room temperature, incubated with avidin-biotin complex for 1 h at room temperature, rinsed with PBS and then treated with 0.5 mg/mL DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)Immunofluorescent analysis of 4% paraformaldehyde fixed Mouse Spleen Tissue labeling SQSTM1 / p62 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-SQSTM1 / p62 antibody (ab91526)All lanes : Anti-SQSTM1 / p62 antibody (ab91526) at 0.5 µg/ml
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate - untreated
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 µg/mL LPS for 3 hours
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 µg/mL LPS for 6 hours
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 µg/mL LPS for 24 hours
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
Raw 264.7 cells were treated with LPS (0.3 μg/mL) for different time period (0-24 hrs). There was an increase in SQSTM1 protein expression overtime after LPS treatment.
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Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab91526)A431 (human epidermoid carcinoma cell line) cells stained for SQSTM1 / p62 (green) using ab91526 at 20 µg/ml in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab91526)Immunofluorescence of SQSTM1 / p62 in Rat Spleen cells using ab91526 at 20 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)Paraffin-embedded human spleen tissue stained for SQSTM1/p62 using ab91526 at 5 µg/ml in immunohistochemical analysis.
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Western blot - Anti-SQSTM1 / p62 antibody (ab91526)Lane 1 : Anti-SQSTM1 / p62 antibody (ab91526) at 1 µg/ml
Lane 2 : Anti-SQSTM1 / p62 antibody (ab91526) at 2 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab91526)Image from Traver MK et al, J Biol Chem. 2011 Sep 2;286(35):30471-80. Epub 2011 Jul 12, Fig 5. DOI 10.1074/jbc.M111.251967 September 2, 2011 The Journal of Biological Chemistry, 286, 30471-30480.ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. -
Western blot - Anti-SQSTM1 / p62 antibody (ab91526)Western blot analysis of SQSTM1 expression in K562 cell lysate with ab91526 at 1µg/ml in (A) the absence and (B) the presence of blocking peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 5 μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)Mouse spleen tissue stained for SQSTM1 / p62 using ab91526 at 2 μg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (206)
ab91526 has been referenced in 206 publications.
- Ishikawa M et al. The neurosteroid allopregnanolone protects retinal neurons by effects on autophagy and GABRs/GABAA receptors in rat glaucoma models. Autophagy 17:743-760 (2021). PubMed: 32070183
- Pesce NA et al. Autophagy Involvement in the Postnatal Development of the Rat Retina. Cells 10:N/A (2021). PubMed: 33477313
- Muralidharan C et al. Pancreatic beta cell autophagy is impaired in type 1 diabetes. Diabetologia 64:865-877 (2021). PubMed: 33515072
- Manganelli V et al. The Role of Cardiolipin as a Scaffold Mitochondrial Phospholipid in Autophagosome Formation: In Vitro Evidence. Biomolecules 11:N/A (2021). PubMed: 33562550
- Wang L et al. miR-4478 sensitizes ovarian cancer cells to irradiation by inhibiting Fus and attenuating autophagy. Mol Ther Nucleic Acids 23:1110-1119 (2021). PubMed: 33664992