Recombinant Anti-SREC-I antibody [EPR3848] (ab92308)
![Western blot - Anti-SREC-I antibody [EPR3848] (ab92308)](https://www.abcam.com/ps/products/92/ab92308/Images/ab92308-107240-anti-srec-i-antibody-epr3848-western-blot.jpg "Western blot - Anti-SREC-I antibody [EPR3848] (ab92308)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3848] to SREC-I
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SREC-I antibody [EPR3848]
See all SREC-I primary antibodies -
Description
Rabbit monoclonal [EPR3848] to SREC-I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SREC-I aa 800-900. The exact sequence is proprietary.
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Positive control
- Human placenta and Human fetal heart lysates; Human lung and spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3848 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. Stronger staining has been observed with polymerised antibodies. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 87 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. Stronger staining has been observed with polymerised antibodies. |
Target
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Function
Mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Mediates heterophilic interactions, suggesting a function as adhesion protein. Plays a role in the regulation of neurite-like outgrowth. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 6 EGF-like domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8578 Human
- Omim: 607873 Human
- SwissProt: Q14162 Human
- Unigene: 647430 Human
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Alternative names
- Acetyl LDL receptor antibody
- Endothelial cells scavenger receptor antibody
- SCARF1 antibody
see all
Images
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Western blot - Anti-SREC-I antibody [EPR3848] (ab92308)All lanes : Anti-SREC-I antibody [EPR3848] (ab92308) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 87 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREC-I antibody [EPR3848] (ab92308)](https://www.abcam.com/ps/products/92/ab92308/Images/ab92308-107239-anti-srec-i-antibody-epr3848-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREC-I antibody [EPR3848] (ab92308)ab92308 at 1/250 dilution staining SREC-I in Human lung tissue by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREC-I antibody [EPR3848] (ab92308)](https://www.abcam.com/ps/products/92/ab92308/Images/ab92308-107238-anti-srec-i-antibody-epr3848-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREC-I antibody [EPR3848] (ab92308)ab92308 at 1/250 dilution staining SREC-I in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-SREC-I antibody [EPR3848] (ab92308)](https://www.abcam.com/ps/products/92/ab92308/Images/ab92308-4-benefits-of-recombinant-antibodies.png)
Anti-SREC-I antibody [EPR3848] (ab92308)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab92308 has been referenced in 3 publications.
- Wicker-Planquart C et al. Molecular and Cellular Interactions of Scavenger Receptor SR-F1 With Complement C1q Provide Insights Into Its Role in the Clearance of Apoptotic Cells. Front Immunol 11:544 (2020). PubMed: 32296440
- Patten DA et al. Prognostic Value and Potential Immunoregulatory Role of SCARF1 in Hepatocellular Carcinoma. Front Oncol 10:565950 (2020). PubMed: 34354939
- Patten DA et al. SCARF-1 promotes adhesion of CD4+T cells to human hepatic sinusoidal endothelium under conditions of shear stress. Sci Rep 7:17600 (2017). PubMed: 29242513