Recombinant Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)
![Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-354759-anti-stc2-antibody-epr-22866-10-western-blot-human-2.jpg "Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10]
See all Stanniocalcin 2/STC-2 primary antibodies -
Description
Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, MCF7, HT-29, 293T, HCT116 and Human breast cancer lysates. IHC-P: Human lung cancer and Human breast cancer tissues. ICC/IF: T-47D and HepG2 cells. Flow Cyt (intra): T-47D and HepG2 cells. IP: T-47D cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22866-10 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
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| WB |
1/1000. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 33 kDa).
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| IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/50.
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| IP |
1/30.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
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WB
1/1000. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 33 kDa). |
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IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/50. |
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IP
1/30. |
Target
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Function
Has an anti-hypocalcemic action on calcium and phosphate homeostasis. -
Tissue specificity
Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Belongs to the stanniocalcin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8614 Human
- Omim: 603665 Human
- SwissProt: O76061 Human
- Unigene: 233160 Human
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Alternative names
- Stanniocalcin 2 antibody
- Stanniocalcin related protein antibody
- Stanniocalcin-2 antibody
see all
Images
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Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)All lanes : Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610) at 1/1000 dilution
Lane 1 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 2 : Human breast cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID:15367391, 25463045, 26361149).
Exposure time: Lane 1: 3 minutes; Lane 2: 48 seconds.
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![Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-354758-anti-stc2-antibody-epr-22866-10-western-blot-human.jpg)
Western blot - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)All lanes : Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 33-35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature, the dual bands caused by different posttranslational modifications (PMID: 15367391, 25463045, 26361149).
Exposure time: Lane 1-2: 48 seconds; Lane 3: 26 seconds; Lane 4: 114 seconds; Lane 5: 15 seconds.
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![Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-7-anti-stc2-antibody-epr22866-10-immunoprecipitation-t47d-human.jpg)
Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)STC2 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with ab255610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255610 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate 10ug
Lane 2: ab255610 IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255610 in T-47D whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-6-anti-stc2-antibody-epr22866-10-flowcytometry-hepg2-human.jpg)
Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-5-anti-stc2-antibody-epr22866-10-flowcytometry-t47d-human.jpg)
Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-4-anti-stc2-antibody-epr22866-10-immunohistochemistry-breast-and-breast-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Immunohistochemical analysis of paraffin-embedded Human breast cancer (A) and its adjacent noncancerous breast tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human breast cancer tissue (A) while weak staining in its adjacent noncancerous breast tissue (B) (PMID: 18492817) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-3-anti-stc2-antibody-epr22866-10-immunohistochemistry-lung-and-lung-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous lung tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous lung tissue (B) (PMID: 25463045) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-2-anti-stc2-antibody-epr22866-10-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-1-anti-stc2-antibody-epr22866-10-immunocytochemistry-t47d-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in T-47D cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Ab255610 anti-STC2 ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
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![Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)](https://www.abcam.com/ps/products/255/ab255610/Images/ab255610-10-benefits-of-recombinant-antibodies.png)
Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (ab255610)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab255610 has been referenced in 1 publication.
- Li J et al. Stanniocalcin-2 promotes cell EMT and glycolysis via activating ITGB2/FAK/SOX6 signaling pathway in nasopharyngeal carcinoma. Cell Biol Toxicol N/A:N/A (2021). PubMed: 33797657