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RecombinantRabMAb

Recombinant Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

Submit a review Submit a question References (2)
Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
  • Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

Overview

  • Product name

    Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free
    See all Stanniocalcin 2/STC-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, MCF7, HT-29, 293T, HCT116 and Human breast cancer lysates. IHC-P: Human lung cancer and Human breast cancer tissues. ICC/IF: T-47D and HepG2 cells. Flow Cyt (intra): T-47D and HepG2 cells. IP: T-47D cells.

  • General notes

    ab261915 is the carrier-free version of ab255610.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab261915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 33 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 33 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Function

    Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

  • Tissue specificity

    Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the stanniocalcin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Stanniocalcin 2 antibody
      • Stanniocalcin related protein antibody
      • Stanniocalcin-2 antibody
      • Stanniocalcin-related protein antibody
      • Stanniocalcin2 antibody
      • STC 2 antibody
      • STC related protein antibody
      • STC-2 antibody
      • STC-related protein antibody
      • STC2 antibody
      • STC2_HUMAN antibody
      • STCRP antibody
      see all

    Images

    • Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      STC2 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with ab255610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255610 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate 10ug

      Lane 2: ab255610 IP in T-47D whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255610 in T-47D whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

    • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610). 

       

       

    • Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Flow Cytometry (Intracellular) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Immunohistochemical analysis of paraffin-embedded Human breast cancer (A) and its adjacent noncancerous breast tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human breast cancer tissue (A) while weak staining in its adjacent noncancerous breast tissue (B) (PMID: 18492817) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous lung tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous lung tissue (B) (PMID: 25463045) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

    • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

    • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in T-47D cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is Ab255610 anti-STC2 ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

    • Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
      Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab261915? Please let us know so that we can cite the reference in this datasheet.

    ab261915 has been referenced in 2 publications.

    • Ortega MA  et al. Abnormal proinflammatory and stressor environmental with increased the regulatory cellular IGF-1/PAPP-A/STC and Wnt-1/β-Catenin canonical pathway in placenta of women with Chronic venous Disease during Pregnancy. Int J Med Sci 18:2814-2827 (2021). PubMed: 34220309
    • Ortega MA  et al. Chronic Venous Disease Patients Showed Altered Expression of IGF-1/PAPP-A/STC-2 Axis in the Vein Wall. Biomed Res Int 2020:6782659 (2020). PubMed: 33381575

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