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    products/primary-antibodies/stat2-antibody-y141-ab32367.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STAT2 antibody [Y141] (ab32367)

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Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Anti-STAT2 antibody [Y141] (ab32367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y141] to STAT2
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

You may also be interested in

Primary
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Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] - BSA and Azide free (ab219728)
Knockout
Product image
Human STAT2 knockout A549 cell lysate (ab257185)
Knockout
Product image
Human STAT2 knockout HeLa cell line (ab261819)

View more associated products

Overview

  • Product name

    Anti-STAT2 antibody [Y141]
    See all STAT2 primary antibodies
  • Description

    Rabbit monoclonal [Y141] to STAT2
  • Host species

    Rabbit
  • Specificity

    This antibody detects both long and short forms of STAT2.
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human STAT2 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: A549, K562, HAP1, Ramos, HeLa and THP1 cell lysates; Mouse and Rat brain tissue lysates ICC/IF: THP-1 cells IHC-P: Human hepatocellular carcinoma and thyroid cancer tissue, Mouse and Rat liver Tissue Flow Cyt (intra): Ramos cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y141
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

Associated products

  • Alternative Versions

    • Anti-STAT2 antibody [Y141] - BSA and Azide free (ab239811)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STAT2 knockout HeLa cell line (ab261819)
    • Human STAT2 knockout A549 cell line (ab267004)
    • Human STAT2 knockout A549 cell line (ab267005)
    • Human STAT2 knockout A549 cell line (ab267006)
  • KO cell lysates

    • Human STAT2 knockout HeLa cell lysate (ab257182)
    • Human STAT2 knockout A549 cell lysate (ab257183)
    • Human STAT2 knockout A549 cell lysate (ab257184)
    • Human STAT2 knockout A549 cell lysate (ab257185)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human STAT2 protein (ab81880)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Flow Cyt (Intra)
1/60.
ICC/IF
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Notes
WB
1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Flow Cyt (Intra)
1/60.
ICC/IF
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Target

  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Target information above from: UniProt accession P52630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6773 Human
    • Entrez Gene: 20847 Mouse
    • Entrez Gene: 288774 Rat
    • Omim: 600556 Human
    • SwissProt: P52630 Human
    • SwissProt: Q9WVL2 Mouse
    • Unigene: 530595 Human
    • Unigene: 293120 Mouse
    • Unigene: 471333 Mouse
    see all
  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Images

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab32367 was shown to react with STAT2 in A549 wild-type cells in western blot with loss of signal observed in STAT2 knockout cell line ab267004 (STAT2 knockout cell lysate ab257183). Wild-type and STAT2 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk before incubation with ab32367 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32367 was shown to react with STAT2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267005 (knockout cell lysate ab257184) was used. Wild-type A549 and STAT2 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32367 was shown to react with STAT2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261819 (knockout cell lysate ab257182) was used. Wild-type HeLa and STAT2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab32367 Anti-STAT2 antibody [Y141] was shown to specifically react with STAT2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267006 (knockout cell lysate ab257185) was used. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STAT2 knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : THP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 98 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32367 (unpurified) observed at 97 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32367 was shown to recognize STAT2 in wild-type HAP1 cells as signal was lost at the expected MW in STAT2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. Ab32367 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)
    Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)

    Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling STAT2 with purified ab32367 at 1/60 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)

    Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling STAT2 with purified ab32367 at 1/50 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/1000 dilution (Purified)

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 98 kDa
    Observed band size: 113,97 kDa why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ab32367 (unpurified). Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-STAT2 antibody [Y141] (ab32367)
    Anti-STAT2 antibody [Y141] (ab32367)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab32367? Please let us know so that we can cite the reference in this datasheet.

ab32367 has been referenced in 2 publications.

  • Qu T  et al. ISG15 induces ESRP1 to inhibit lung adenocarcinoma progression. Cell Death Dis 11:511 (2020). PubMed: 32641707
  • Yin D  et al. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124. Cell Physiol Biochem 50:2188-2202 (2018). PubMed: 30415256

Customer reviews and Q&As

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Western blot abreview for Anti-STAT2 antibody [Y141]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

Submitted Oct 20 2020

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