Name: Anti-STAT3 antibody [9D8]
SKU: ab119352
Rating: 4 (11 reviews)

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Western blot - Anti-STAT3 antibody [9D8] (ab119352)

Key features and details

Mouse monoclonal [9D8] to STAT3
Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
Reacts with: Mouse, Rat, Human, African green monkey
Isotype: IgG

Product name

Anti-STAT3 antibody [9D8]
See all STAT3 primary antibodies
Description

Mouse monoclonal [9D8] to STAT3
Host species

Mouse
Specificity

Detects STAT3 from Human, murine, monkey and rat samples.
Tested applications

Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human, African green monkey
Immunogen

Recombinant fragment, corresponding to amino acids 665-770 of Human STAT3.
Positive control
- HepG2, HeLa, PC-12, A549, 293T, Jurkat, A431, U2OS, MCF7, A549, K562, NIH3T3, C2C12, and NRK whole cell lysates; U2OS lysate from STAT3 SMART pool siRNA transfected; Mouse lung tissue lysate; HeLa cells; Human pancreas, brain tumour, colon cancer and stomach cancer tissues Flow Cyt: HeLa cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

9D8
Isotype

IgG
Research areas

Assay kits
- STAT3 Transcription Factor Assay Kit (Colorimetric) (ab207229)
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG - Isotype Control (ab37355)
Positive Controls
Recombinant Protein
- Recombinant Human STAT3 protein (ab43618)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		Use 0.1µg for 10⁶ cells.
ICC/IF		1/100.
WB	(2)	1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP		Use at 2 µg/mg of lysate.
IHC-P	(5)	1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Notes
Flow Cyt Use 0.1µg for 10⁶ cells.
ICC/IF 1/100.
WB 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP Use at 2 µg/mg of lysate.
IHC-P 1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Function

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease

Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset
Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications

Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Cellular localization

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Target information above from: UniProt accession P40763 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
- SwissProt: P52631 Rat
- Unigene: 463059 Human
- Unigene: 249934 Mouse
- Unigene: 473190 Mouse
- Unigene: 10247 Rat
see all
Alternative names
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
- ADMIO antibody
- APRF antibody
- AW109958 antibody
- DNA binding protein APRF antibody
- FLJ20882 antibody
- HIES antibody
- MGC16063 antibody
- Signal transducer and activator of transcription 3 (acute phase response factor) antibody
- Signal transducer and activator of transcription 3 antibody
- STAT 3 antibody
- Stat3 antibody
- STAT3_HUMAN antibody
see all

Western blot - Anti-STAT3 antibody [9D8] (ab119352)

All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution

Lane 1 : U2OS lysate from non-targeting control
Lane 2 : U2OS lysate from STAT3 SMART pool siRNA transfected

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 88 kDa

4-20% Tris-HCl polyacrylamide gel
Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [9D8] (ab119352)

Immunofluorescent analysis of STAT3 using ab119352 at 1/100 dilution (shown in green) in HeLa cells (formalin fixed cells permeabilized with 0.1% Triton X-100) using a DyLight 488 goat-anti-mouse secondary antibody (ab96879) at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.
Flow Cytometry - Anti-STAT3 antibody [9D8] (ab119352)

Overlay histogram showing HeLa cells stained with ab119352 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119352, 0.1μg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (ab37355, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-STAT3 antibody [9D8] (ab119352)

All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 8 : Mouse lung tissue lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 88 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Western blot - Anti-STAT3 antibody [9D8] (ab119352)

All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lane 5 : U2OS cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : A549 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : COS7 cell lysate
Lane 10 : NIH3T3 cell lysate
Lane 11 : C2C12 cell lysate
Lane 12 : NRK cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : goat anti-mouse-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 88 kDa

4-20% Tris-HCl polyacrylamide gel
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

ab119352, at 1/1600 dilution, staining STAT3 in Human pancreas tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
Western blot - Anti-STAT3 antibody [9D8] (ab119352)

Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution + HepG2 total lysate at 25 µg

Secondary
goat anti-mouse-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 88 kDa

4-20% Tris-HCl polyacrylamide gel
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

ab119352, at 1/1600 dilution, staining STAT3 in Human brain tumor tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
Immunoprecipitation - Anti-STAT3 antibody [9D8] (ab119352)

Immunoprecipitation of STAT3 was performed on HepG2 cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of ab119352 overnight at 4°C. The immune-complex was captured, washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1% Tween for at least 1 hour. Membranes were then probed with ab119352 at a dilution of 1/5000 overnight at 4°C. Membranes were washed in TBST and probed with IP detection reagent at a dilution of 1/2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

ab119352, at 1/1600 dilution, staining STAT3 in Human colon cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

ab119352, at 1/1600 dilution, staining STAT3 in Human stomach cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab119352? Please let us know so that we can cite the reference in this datasheet.

ab119352 has been referenced in 181 publications.

Zheng X et al. Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637. Bioengineered 13:440-454 (2022). PubMed: 34967281
Zhang Z et al. Rifaximin protects SH-SY5Y neuronal cells from iron overload-induced cytotoxicity via inhibiting STAT3/NF-κB signaling. Cell Biol Int 46:1062-1073 (2022). PubMed: 35143099
Xia J et al. Versatile ginsenoside Rg3 liposomes inhibit tumor metastasis by capturing circulating tumor cells and destroying metastatic niches. Sci Adv 8:eabj1262 (2022). PubMed: 35148178
Wang Y et al. Curcumol Attenuates Endometriosis by Inhibiting the JAK2/STAT3 Signaling Pathway. Med Sci Monit 28:e934914 (2022). PubMed: 35279667
Chai Y et al. RING-Finger Protein 6 promotes Drug Resistance in Retinoblastoma via JAK2/STAT3 Signaling Pathway. Pathol Oncol Res 28:1610273 (2022). PubMed: 35369571

View all Publications for this product

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Application

ChIP

Sample

Human Cell lysate - nuclear (Glioma cell lines U87MG and U251MG)

Negative control

non specific IP by IgG

Specification

Glioma cell lines U87MG and U251MG

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde in PBS at room

Positive control

Discontinued STAT3 antibody sc482X OSMR promoter, a gene previously reported to be regulated by STAT3 (eg/ PMID: 29142070)

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Julie Thoms

Verified customer

Submitted Dec 24 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate ph 6

Permeabilization

Specification

brain tissue

Blocking step

Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Markus Kipp

Verified customer

Submitted Aug 21 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (tumor)

Permeabilization

Specification

tumor

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 30 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Dog Tissue sections (artery)

Permeabilization

Specification

artery

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 30 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (tumor xenograft)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

tumor xenograft

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

lung

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

Liver

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (stomach)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

stomach

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Lung)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

22 µg

Specification

Lung

Blocking step

Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Pete Serbedzija

Verified customer

Submitted Jul 28 2016

Application

Immunohistochemistry (Frozen sections)

Blocking step

0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C

Sample

Mouse Tissue sections (mouse embryonic spinal cord E11)

Specification

mouse embryonic spinal cord E11

Permeabilization

Yes - 0.25%TritonX100

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2014

1-10 of 12 Abreviews or Q&A

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Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Assay kits

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (181)

Customer reviews and Q&As

ChIP abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

Western blot abreview for Anti-STAT3 antibody [9D8]

Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

Post-translational
modifications