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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STAT3 antibody [EPR787Y] (ab68153)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (204)

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Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Flow Cytometry (Intracellular) - Anti-STAT3 antibody [EPR787Y] (ab68153)
  • Anti-STAT3 antibody [EPR787Y] (ab68153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR787Y] to STAT3
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

You may also be interested in

ELISA
Product image
Human PSA ELISA Kit (KLK3) (ab113327)
Primary
Product image
HRP Anti-STAT3 antibody [EPR787Y] (ab194307)
Knockout
Product image
Human STAT3 knockout HeLa cell line (ab255436)

View more associated products

Overview

  • Product name

    Anti-STAT3 antibody [EPR787Y]
    See all STAT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR787Y] to STAT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat and mouse heart tissue lysates; Human, mouse, and rat brain tissue lysates; Rat kidney tissue lysate; HeLa, HAP1, A431, HaCaT, NIH/3T3, C2C12, and Raji cell lysates. IHC-P: Human brain tissue. ICC/IF: HeLa cells. Flow Cyt (intra): Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR787Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Alternative Versions

    • Anti-STAT3 antibody [EPR787Y] - BSA and Azide free (ab171359)
    • HRP Anti-STAT3 antibody [EPR787Y] (ab194307)
  • Assay kits

    • STAT3 Transcription Factor Assay Kit (Colorimetric) (ab207229)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STAT3 knockout HeLa cell line (ab255436)
  • KO cell lysates

    • Human STAT3 knockout HeLa cell lysate (ab263797)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
  • Recombinant Protein

    • Recombinant Human STAT3 protein (ab43618)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30 - 1/50.
ICC/IF (1)
1/200.

For unpurified use at 1/140.

WB (7)
1/1000 - 1/2000. Detects a band of approximately 75, 88 kDa (predicted molecular weight: 88 kDa).
IHC-P (1)
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/140.

Notes
Flow Cyt (Intra)
1/30 - 1/50.
ICC/IF
1/200.

For unpurified use at 1/140.

WB
1/1000 - 1/2000. Detects a band of approximately 75, 88 kDa (predicted molecular weight: 88 kDa).
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/140.

Application notes
Is unsuitable for IP.

Target

  • Function

    Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in disease

    Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Target information above from: UniProt accession P40763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6774 Human
    • Entrez Gene: 20848 Mouse
    • Entrez Gene: 25125 Rat
    • Omim: 102582 Human
    • SwissProt: P40763 Human
    • SwissProt: P42227 Mouse
    • SwissProt: P52631 Rat
    • Unigene: 463059 Human
    • Unigene: 249934 Mouse
    • Unigene: 473190 Mouse
    • Unigene: 10247 Rat
    see all
  • Alternative names

    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Images

  • Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    All lanes : Anti-STAT3 antibody [EPR787Y] (ab68153) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT3 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 92 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab68153 observed at 92 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab68153 was shown to react with STAT3 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255436 (knockout cell lysate ab263797) was used. Wild-type HeLa and STAT3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab68153 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [EPR787Y] (ab68153)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labelling STAT3 with purified ab68153 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    All lanes : Anti-STAT3 antibody [EPR787Y] (ab68153) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 5 : Human brain lysate
    Lane 6 : Mouse brain lysate
    Lane 7 : Rat brain lysate
    Lane 8 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 88 kDa
    Observed band size: 92 kDa why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST.

    Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

  • Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Western blot - Anti-STAT3 antibody [EPR787Y] (ab68153)
    All lanes : Anti-STAT3 antibody [EPR787Y] (ab68153) at 1/500 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STAT3 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 88 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab68153 observed at 92 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Ab68153 detected the expected band for STAT3 in wild-type cells along with additional cross-reactive bands. The band was not seen in STAT3 knockout HAP1 cells. Wild-type and STAT3 knockout samples were subjected to SDS-PAGE. Ab68153 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [EPR787Y] (ab68153)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling STAT3 (green) with purified ab68153 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry (Intracellular) - Anti-STAT3 antibody [EPR787Y] (ab68153)
    Flow Cytometry (Intracellular) - Anti-STAT3 antibody [EPR787Y] (ab68153)

    ab68153 stainingSTAT3in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Anti-STAT3 antibody [EPR787Y] (ab68153)
    Anti-STAT3 antibody [EPR787Y] (ab68153)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (204)

Publishing research using ab68153? Please let us know so that we can cite the reference in this datasheet.

ab68153 has been referenced in 204 publications.

  • Tang J  et al. MiR-125b participates in the occurrence of preeclampsia by regulating the migration and invasion of extravillous trophoblastic cells through STAT3 signaling pathway. J Recept Signal Transduct Res 41:202-208 (2021). PubMed: 32787544
  • Wang L  et al. 13R,20-Dihydroxydocosahexaenoic Acid, a Novel Dihydroxy- DHA Derivative, Inhibits Breast Cancer Stemness through Regulation of the Stat3/IL-6 Signaling Pathway by Inducing ROS Production. Antioxidants (Basel) 10:N/A (2021). PubMed: 33804152
  • Silva Raju J  et al. Prognostic Value of TNFR2 and STAT3 among High-Grade Serous Ovarian Cancer Survivors According to Platinum Sensitivity. Diagnostics (Basel) 11:N/A (2021). PubMed: 33809542
  • Farini A  et al. Defective dystrophic thymus determines degenerative changes in skeletal muscle. Nat Commun 12:2099 (2021). PubMed: 33833239
  • Xu Z  et al. Astragaloside IV Protects 6-Hydroxydopamine-Induced SH-SY5Y Cell Model of Parkinson's Disease via Activating the JAK2/STAT3 Pathway. Front Neurosci 15:631501 (2021). PubMed: 33833662
View all Publications for this product

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

Submitted Oct 19 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (C42B Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
C42B Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [EPR787Y]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (CT26-WT tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
CT26-WT tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 07 2016

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
1e+006 cells
Specification
HeLa
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 12 2016

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Colon)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
1e+006 cells
Specification
Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 12 2016

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human normal oesopheal cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
10 nM PMA
Specification
human normal oesopheal cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 27 2015

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 06 2015

Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 01 2014

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15% TRIS-Glycine SDS PAGE)
Sample
Mouse Tissue lysate - whole (1) mouse embryonic spinal cord E11, 2) HEK293H)
Specification
1) mouse embryonic spinal cord E11, 2) HEK293H
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2014

Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4–15%)
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 09 2014

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