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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STAT5b antibody [EPR16671] (ab178941)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (12)

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Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
  • ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Anti-STAT5b antibody [EPR16671] (ab178941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16671] to STAT5b
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra), ChIP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Recombinant Human STAT5b protein (ab182829)
Primary
Product image
Anti-STAT5a antibody [E289] (ab32043)

View more associated products

Overview

  • Product name

    Anti-STAT5b antibody [EPR16671]
    See all STAT5b primary antibodies
  • Description

    Rabbit monoclonal [EPR16671] to STAT5b
  • Host species

    Rabbit
  • Specificity

    ab178941 shows no cross reactivity with STAT5a.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra), ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, Jurkat, Daudi whole cell lysates. C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and Rat brain, heart, kidney and spleen lysates. Human fetal heart, kidney and spleen lysates. IHC-P: Rat colon, Mouse spleen and Human spleen tissues. ICC/IF: HeLa cells. IP: K562 whole cell extract. ChIP: T-47D cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16671
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (ab199767)
    • Alexa Fluor® 647 Anti-STAT5b antibody [EPR16671] (ab199846)
    • Anti-STAT5b antibody [EPR16671] - BSA and Azide free (ab240211)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human STAT5b protein (ab182829)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40.
Flow Cyt (Intra)
1/40.
ChIP
Use at an assay dependent concentration.
Notes
WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40.
Flow Cyt (Intra)
1/40.
ChIP
Use at an assay dependent concentration.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • Involvement in disease

    Growth hormone insensitivity with immunodeficiency
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession P51692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6777 Human
    • Entrez Gene: 20851 Mouse
    • Entrez Gene: 25126 Rat
    • Omim: 604260 Human
    • SwissProt: P51692 Human
    • SwissProt: P42232 Mouse
    • SwissProt: P52632 Rat
    • Unigene: 595276 Human
    • Unigene: 34064 Mouse
    • Unigene: 54486 Rat
    see all
  • Alternative names

    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Images

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT5B knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 0.2 µg/ml

    Performed under reducing conditions.

    Predicted band size: 90 kDa



    ab178941 was shown to react with STAT5B in wild-type HeLa cells in Western blot with loss of signal observed in STAT5B knockout cell line ab266006 (STAT5B knockout cell lysate ab257710). Wild-type HeLa and STAT5B knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab178941 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2μg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT5B knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab178941 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab178941 Anti-STAT5b antibody [EPR16671] was shown to specifically react with STAT5b in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266006 (knockout cell lysate ab257710) was used. Wild-type and STAT5b knockout samples were subjected to SDS-PAGE. ab178941 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 20000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunoprecipitation of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract using ab178941 at 1/40 dilution. Western blot detection of STAT5b utilised ab178941 at 1/2000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and dilution buffer was 5% NFDM/TBST.

  • ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)
    ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)

    Chromatin was prepared from T-47D (starved overnight) treated with Prolactin(10nM 30min) and T-47D(starved overnight) non-treated cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of ab178941 (red), or 5 μg of rabbit normal IgG ab172730 (gray) and 20 μL of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers are from PMID: 15686596.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling STAT5b with ab178941 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). Nuclear and cytoplasm staining is detected. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). 

     

    The negative controls are as follows;

    1. ab178941 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) ar 1/200 dilution.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes of Human spleen is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)

    ab178941 staining STAT5bin the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/40. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 4 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat kidney lysates
    Lane 8 : Rat spleen lysates
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human fetal kidney lysates
    Lane 3 : Human fetal spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Lane 1 : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/1000 dilution
    Lane 2 : Anti-STAT5a antibody [E289] (ab32043) at 1/5000 dilution

    All lanes : STAT5a recombinant protein

    Developed using the ECL technique.

    Predicted band size: 90 kDa



    WB showing no cross reactivity with STAT5a.

  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)

    Cross Immunoprecipitation of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract showing no cross reactivity with STAT5a. Protein captured by anti-STAT5a antibody (ab32042) was detected by the same antibody in WB (image 1) but not by anti-STAT5b, ab178941 (image 2).

     

    For WB detection, ab178941 was used at a 1/2000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at a 1/1000 dilution. The blocking and dilution buffer was 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes of Mouse spleen is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes and weak nucleus staining on gland epithelium of colon is detected. The negative control utilised PBS insead of primary antibody and the slide is counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Flow Cytometry (Intracellular) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Intracellular Flow Cytometry analysis of 2% paraformaldehyde K562 (Human chronic myelogenous leukemia cells from bone marrow) cellslabeling STAT5b with ab178941 at 1/60 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). 

     

     

  • Anti-STAT5b antibody [EPR16671] (ab178941)
    Anti-STAT5b antibody [EPR16671] (ab178941)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (12)

Publishing research using ab178941? Please let us know so that we can cite the reference in this datasheet.

ab178941 has been referenced in 12 publications.

  • Meyer LK  et al. Glucocorticoids paradoxically facilitate steroid resistance in T cell acute lymphoblastic leukemias and thymocytes. J Clin Invest 130:863-876 (2020). PubMed: 31687977
  • Zhang Y  et al. Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5. Environ Sci Pollut Res Int 27:3837-3848 (2020). PubMed: 31732953
  • Xiang Y  et al. MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function. Cell Commun Signal 18:107 (2020). PubMed: 32646440
  • Zhang G  et al. Testosterone propionate activated the Nrf2-ARE pathway in ageing rats and ameliorated the age-related changes in liver. Sci Rep 9:18619 (2019). PubMed: 31819135
  • Zhang Y  et al. Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells. Ecotoxicol Environ Saf 184:109611 (2019). PubMed: 31491605
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-STAT5b antibody [EPR16671]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HEPG2)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
HEPG2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

Submitted Oct 20 2020

Western blot abreview for Anti-STAT5b antibody [EPR16671]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate cancer, DU-145)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
prostate cancer, DU-145
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 14 2019

Western blot abreview for Anti-STAT5b [EPR16671] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
monocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Matija Hedl

Verified customer

Submitted Mar 02 2016

Western blot abreview for Anti-STAT5b [EPR16671] antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (dendritic cells)
Specification
dendritic cells
Treatment
50 ng/ml GM-CSF (6hrs) or LPS 1´g/ml(24hrs)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. A Amrani

Verified customer

Submitted Oct 27 2014

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