Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-STAT6 antibody [EPR18278-222] (ab217998)

Submit a review Submit a question
Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)
  • Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
  • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (ab217998)
  • Anti-STAT6 antibody [EPR18278-222] (ab217998)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18278-222] to STAT6
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-STAT6 antibody [EPR18278-222]
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18278-222] to STAT6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human thymus lysate; HeLa, P388D1, Ramos, THP-1, Daudi, Raji and A20 whole cell lysates; Mouse and rat spleen lysates. ICC/IF: Raji and Daudi cells. IP: Raji whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500.
WB
1/1000. Detects a band of approximately 110, 81, 75 kDa (predicted molecular weight: 94 kDa).
IP
Use at an assay dependent concentration.
Notes
ICC/IF
1/500.
WB
1/1000. Detects a band of approximately 110, 81, 75 kDa (predicted molecular weight: 94 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate
      Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
      Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 6 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
      Lane 7 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,2 and 3: 8 seconds; Lane 4 and 5: 2 seconds; Lane 6 and 7: 1 second.

      The molecular weight observed is consistent with what has been described in the literature (PMID 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

    • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)

      Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID: 11781246] [PMID: 23575898].

      The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (ab217998)

      Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Rat spleen tissue lysate
      Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1:10 seconds; Lane 2:3 seconds; Lane 3:5 seconds.

    • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (ab217998)

      STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate, 10 µg (Input).

      Lane 2: ab217998 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

    • Anti-STAT6 antibody [EPR18278-222] (ab217998)
      Anti-STAT6 antibody [EPR18278-222] (ab217998)

    References (0)

    Publishing research using ab217998? Please let us know so that we can cite the reference in this datasheet.

    ab217998 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab217998.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com