Recombinant Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Intracellular Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT6 with purified ab32520 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520). 

Immunofluorescence staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells with purified ab32520 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32520 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

ab32520 (purified) at 1/20 immunoprecipitating STAT6 in 10 μg NIH/3T3 (mouse embyro fibroblast cell line; Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Clone YE361 (ab215995) has been successfully conjugated by Abcam. This image was generated using Anti-STAT6 antibody [YE361] (Alexa Fluor® 647). Please refer to ab196928 for protocol details.

ab196928 staining STAT6 in HACAT cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196928 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Clone YE361 (ab215995) has been successfully conjugated by Abcam. This image was generated using Anti-STAT6 antibody [YE361] (Alexa Fluor® 488). Please refer to ab196478 for protocol details.

ab196478 staining STAT6 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196478 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Clone YE361 (ab215995) has been successfully conjugated by Abcam. This image was generated using Anti-STAT6 antibody [YE361] (PE). Please refer to ab223917 for protocol details. 

Overlay histogram showing NIH3T3 cells stained with ab223917 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223917, 1/1000 dilution) for 30 min at 22°C. 

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. 

This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions. 

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

This IHC data was generated using the same anti-STAT6 antibody clone, YE361, in a different buffer formulation (cat# ab32520).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling STAT6 with unpurified ab32520 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence analyis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling STAT6 with unpurified ab32520 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human solitary fibrous tumor tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32520).