Recombinant Anti-STING antibody [SP339] KO Tested (ab227705)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP339] to STING
Suitable for: Flow Cyt (Intra), IHC-P, WB
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-STING antibody [SP339]
See all STING primary antibodies
Description

Rabbit monoclonal [SP339] to STING
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Cow, Pig
Immunogen

Synthetic peptide within Human TMEM173 aa 150-350 (C terminal). The exact sequence is proprietary.
Database link: Q86WV6
Positive control
- WB: THP-1 and Wild-type THP-1 cell lysate. IHC-P: Human tonsil, colon, colon carcinoma, thymus, lymph node, lung, HK lymphoma, lung adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma and skin squamous cell carcinoma tissues. Flow Cyt: THP-1 cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP339
Isotype

IgG
Research areas

Alternative Versions
- Anti-STING antibody [SP339] - BSA and Azide free (ab238796)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human TMEM173 knockout THP-1 cell line (ab270493)
KO cell lysates
- Human TMEM173 knockout THP-1 cell lysate (ab270516)
Positive Controls
- THP-1 whole cell lysate (ab7913)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/20 - 1/400. Primary incubation for 30 minutes at 4°C.
IHC-P		1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature.
WB		1/400. Predicted molecular weight: 42 kDa. Primary incubation for 1 hour at room temperature.

Notes
Flow Cyt (Intra) 1/20 - 1/400. Primary incubation for 30 minutes at 4°C.
IHC-P 1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature.
WB 1/400. Predicted molecular weight: 42 kDa. Primary incubation for 1 hour at room temperature.

Function

Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
Tissue specificity

Ubiquitously expressed.
Sequence similarities

Belongs to the TMEM173 family.
Post-translational
modifications

Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
Cellular localization

Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
Target information above from: UniProt accession Q86WV6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 340061 Human
- Omim: 612374 Human
- SwissProt: Q86WV6 Human
- Unigene: 379754 Human
Alternative names
- endoplasmic reticulum IFN stimulator antibody
- Endoplasmic reticulum interferon stimulator antibody
- ERIS antibody
- FLJ38577 antibody
- hMITA antibody
- hSTING antibody
- Mediator of IRF3 activation antibody
- MITA antibody
- Mitochondrial mediator of IRF3 activation antibody
- MPYS antibody
- N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
- NET23 antibody
- Stimulator of interferon genes antibody
- Stimulator of interferon genes protein antibody
- STING antibody
- TM173_HUMAN antibody
- Tmem173 antibody
- Transmembrane protein 173 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.
Western blot - Anti-STING antibody [SP339] (ab227705)

All lanes : Anti-STING antibody [SP339] (ab227705) at 1/400 dilution

Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : TMEM173 knockout THP-1 cell lysate
Lane 3 : Human Tonsil tissue lysate
Lane 4 : Human Thymus tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 42 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab227705 observed at 37 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab227705 was shown to react with TMEM173 in wild-type THP-1 cells in Western blot with loss of signal observed in TMEM173 knockout cell line ab270493 (TMEM173 knockout cell lysate ab270516). Wild-type THP-1 and TMEM173 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab227705 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-STING antibody [SP339] (ab227705)

Anti-STING antibody [SP339] (ab227705) at 1/400 dilution + THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227705 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (ab227705)

Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling TMEM173 with purified ab227705 at 1/20 dilution (12.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry (Intracellular) - Anti-STING antibody [SP339] (ab227705)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227705 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [SP339] (ab227705)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.
Anti-STING antibody [SP339] (ab227705)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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ab227705 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As

Post-translational
modifications