Anti-STRBP antibody (ab237682)
Key features and details
- Rabbit polyclonal to STRBP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STRBP antibody
See all STRBP primary antibodies -
Description
Rabbit polyclonal to STRBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human STRBP aa 201-500.
Database link: Q96SI9 -
Positive control
- WB: HeLa and PC-3 whole cell lysates. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
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| ICC/IF |
1/50 - 1/200.
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 74 kDa. |
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ICC/IF
1/50 - 1/200. |
Target
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Function
Involved in spermatogenesis and sperm function. Plays a role in regulation of cell growth. Binds to double-stranded DNA and RNA. Binds most efficiently to poly(I:C) RNA than to poly(dI:dC) DNA. Binds also to single-stranded poly(G) RNA. Binds non-specifically to the mRNA PRM1 3'-UTR and adenovirus VA RNA. -
Sequence similarities
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
Cellular localization
Cytoplasm. Microtubule-associated that localizes to the manchette in developing spermatids. - Information by UniProt
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Database links
- Entrez Gene: 55342 Human
- Omim: 611138 Human
- SwissProt: Q96SI9 Human
- Unigene: 287659 Human
- Unigene: 694157 Human
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Alternative names
- Spermatid perinuclear RNA-binding protein antibody
- STRBP antibody
- STRBP_HUMAN antibody
Images
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Western blot - Anti-STRBP antibody (ab237682)All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-STRBP antibody (ab237682)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for STRBP (green) using ab237682 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237682 has not yet been referenced specifically in any publications.