Recombinant Anti-Strep-tag II antibody [EPR12666] (ab180957)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12666] to Strep-tag II
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Strep-tag II antibody [EPR12666]
See all Strep-tag II primary antibodies -
Description
Rabbit monoclonal [EPR12666] to Strep-tag II -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Strep-tag II protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12666 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 16-100 kDa.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 16-100 kDa. |
Target
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Relevance
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
Alternative names
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
Images
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All lanes : Anti-Strep-tag II antibody [EPR12666] (ab180957) at 1/1000 dilution (Unpurified)
Lane 1 : Strep-tag II protein at 0.01 µg
Lane 2 : Strep-tag II protein at 0.005 µg
Lane 3 : Strep-tag II protein at 0.001 µg
Lane 4 : Strep-tag II protein at 0.0005 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab180957 has been referenced in 5 publications.
- Esposito E et al. Mitotic checkpoint gene expression is tuned by codon usage bias. EMBO J 41:e107896 (2022). PubMed: 35811551
- De Caluwé L et al. The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells. Front Microbiol 12:615165 (2021). PubMed: 33717005
- Tian M et al. GluN2A and GluN2B NMDA receptors use distinct allosteric routes. Nat Commun 12:4709 (2021). PubMed: 34354080
- Nesterenko PA et al. HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses. Cell Rep 37:110167 (2021). PubMed: 34919800
- Gutierrez-Escribano P et al. A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules. Sci Adv 5:eaay6804 (2019). PubMed: 31807710