Anti-Stromal interaction molecule 1 antibody [5A2] (ab57834)
Key features and details
- Mouse monoclonal [5A2] to Stromal interaction molecule 1
- Suitable for: WB, IHC-P, ICC, Flow Cyt, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Stromal interaction molecule 1 antibody [5A2]
See all Stromal interaction molecule 1 primary antibodies -
Description
Mouse monoclonal [5A2] to Stromal interaction molecule 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC, Flow Cyt, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 24-686 of Human Stromal interaction molecule 1
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General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5A2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab57834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| IP | (2) |
Use at an assay dependent concentration.
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 77 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
Tissue specificity
Ubiquitously expressed in various human primary cells and tumor cell lines. -
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
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Database links
- Entrez Gene: 6786 Human
- Omim: 605921 Human
- SwissProt: Q13586 Human
- Unigene: 501735 Human
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Alternative names
- D11S4896E antibody
- GOK antibody
- OTTHUMP00000164512 antibody
see all
Images
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Western blot - Anti-Stromal interaction molecule 1 antibody (ab57834)
Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab57834)Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab57834)ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab57834)This image is courtesy of an anonymous Abreviewab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Stromal interaction molecule 1 antibody (ab57834)Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab57834 has been referenced in 12 publications.
- Ticci C et al. Expanding the clinical and genetic spectrum of pathogenic variants in STIM1. Muscle Nerve 64:567-575 (2021). PubMed: 34368974
- Uysal-Onganer P et al. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 32098295
- Guo X et al. Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome. Acta Biochim Biophys Sin (Shanghai) 52:207-210 (2020). PubMed: 31942931
- Yang J et al. Transmembrane protein 66 attenuates neointimal hyperplasia after carotid artery injury by SOCE inactivation. Mol Med Rep 20:1436-1442 (2019). PubMed: 31173198
- Ge C et al. Knockdown of STIM1 expression inhibits non-small-cell lung cancer cell proliferation in vitro and in nude mouse xenografts. Bioengineered 10:425-436 (2019). PubMed: 31564210
