Recombinant Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
![Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-320326-anti-stromal-interaction-molecule-1-antibody-epr3414-western-blot.jpg "Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Stromal interaction molecule 1 antibody [EPR3414]
See all Stromal interaction molecule 1 primary antibodies -
Description
Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3414 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
Tissue specificity
Ubiquitously expressed in various human primary cells and tumor cell lines. -
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
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Database links
- Entrez Gene: 6786 Human
- Entrez Gene: 20866 Mouse
- Entrez Gene: 361618 Rat
- Omim: 605921 Human
- SwissProt: Q13586 Human
- SwissProt: P70302 Mouse
- SwissProt: P84903 Rat
- Unigene: 501735 Human
see all -
Alternative names
- D11S4896E antibody
- GOK antibody
- OTTHUMP00000164512 antibody
see all
Images
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Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. Ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-121528-anti-stromal-interaction-molecule-1-antibody-epr3414-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-121529-anti-stromal-interaction-molecule-1-antibody-epr3414-western-blot.jpg)
Western blot - Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution
Lane 1 : SH SY5Y cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa -
![Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)](https://www.abcam.com/ps/products/108/ab108994/Images/ab108994-4-benefits-of-recombinant-antibodies.png)
Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab108994 has been referenced in 15 publications.
- Zheng CB et al. Ang II Promotes Cardiac Autophagy and Hypertrophy via Orai1/STIM1. Front Pharmacol 12:622774 (2021). PubMed: 34079454
- Garcia SM et al. Coupling of store-operated calcium entry to vasoconstriction is acid-sensing ion channel 1a dependent in pulmonary but not mesenteric arteries. PLoS One 15:e0236288 (2020). PubMed: 32702049
- Moon H et al. Crbn modulates calcium influx by regulating Orai1 during efferocytosis. Nat Commun 11:5489 (2020). PubMed: 33127885
- Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754
- Zhou J et al. Identification of chemoresistance-related mRNAs based on gemcitabine-resistant pancreatic cancer cell lines. Cancer Med 9:1115-1130 (2020). PubMed: 31823522