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    products/primary-antibodies/stub1chip-antibody-epr4447-bsa-and-azide-free-ab238966.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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RecombinantRabMAb

Recombinant Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
  • Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
  • Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
  • Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4447] to STUB1/CHIP - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free
    See all STUB1/CHIP primary antibodies
  • Description

    Rabbit monoclonal [EPR4447] to STUB1/CHIP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skeletal muscle tissue.
  • General notes

    ab238966 is the carrier-free version of ab134064.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4447
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
    • PE Anti-STUB1 / CHIP antibody [EPR4447] (ab303131)
    • APC Anti-STUB1 / CHIP antibody [EPR4447] (ab303132)
    • HRP Anti-STUB1 / CHIP antibody [EPR4447] (ab303133)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent concentration.
IHC-P
1/100.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IP
Use at an assay dependent concentration.
IHC-P
1/100.

Target

  • Function

    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity

    Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 3 TPR repeats.
    Contains 1 U-box domain.
  • Domain

    The TPR domain is essential for ubiquitination mediated by UBE2D1.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9UNE7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10273 Human
    • Entrez Gene: 56424 Mouse
    • Entrez Gene: 287155 Rat
    • Omim: 607207 Human
    • SwissProt: Q9UNE7 Human
    • SwissProt: Q9WUD1 Mouse
    • Unigene: 592081 Human
    • Alternative names

      • Antigen NY CO 7 antibody
      • Antigen NY-CO-7 antibody
      • C terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70 interacting protein antibody
      • Carboxy terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70p interacting protein antibody
      • CHIP antibody
      • CHIP_HUMAN antibody
      • CLL associated antigen KW 8 antibody
      • CLL-associated antigen KW-8 antibody
      • E3 ubiquitin protein ligase CHIP antibody
      • E3 ubiquitin-protein ligase CHIP antibody
      • Heat shock protein A binding protein 2 (c terminal) antibody
      • HSPABP2 antibody
      • NY CO 7 antibody
      • PP1131 antibody
      • SDCCAG7 antibody
      • Serologically defined colon cancer antigen 7 antibody
      • STIP1 homology and U Box containing protein 1 antibody
      • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
      • STIP1 homology and U box-containing protein 1 antibody
      • STUB 1 antibody
      • STUB1 antibody
      • UBOX 1 antibody
      • UBOX1 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
      Flow Cytometry (Intracellular) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

      Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling STUB1/CHIP with purified ab134064 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064). 

       

       

    • Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
      Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

      ab134064 (purified) at 1/120 immunoprecipitating STUB1/CHIP in 10 μg HeLa (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).

    • Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
      Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

      Immunofluorescence staining of SH-SY5Y cells with purified ab134064 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab134064 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

      Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling STUB1/CHIP with unpurified ab134064 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab134064).

    • Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
      Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab238966? Please let us know so that we can cite the reference in this datasheet.

    ab238966 has not yet been referenced specifically in any publications.

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