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RecombinantRabMAb

Recombinant Anti-STUB1/CHIP antibody [EPR4448] (ab109103)

Reviews (2) Submit a question References (3)
Western blot - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
  • Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
  • Anti-STUB1/CHIP antibody [EPR4448] (ab109103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4448] to STUB1/CHIP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-STUB1/CHIP antibody [EPR4448]
    See all STUB1/CHIP primary antibodies

  • Description

    Rabbit monoclonal [EPR4448] to STUB1/CHIP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF7, ECV304, and 293T cell lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.

  • Tissue specificity

    Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 3 TPR repeats.
    Contains 1 U-box domain.

  • Domain

    The TPR domain is essential for ubiquitination mediated by UBE2D1.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen NY CO 7 antibody
      • Antigen NY-CO-7 antibody
      • C terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70 interacting protein antibody
      • Carboxy terminus of Hsp70-interacting protein antibody
      • Carboxy terminus of Hsp70p interacting protein antibody
      • CHIP antibody
      • CHIP_HUMAN antibody
      • CLL associated antigen KW 8 antibody
      • CLL-associated antigen KW-8 antibody
      • E3 ubiquitin protein ligase CHIP antibody
      • E3 ubiquitin-protein ligase CHIP antibody
      • Heat shock protein A binding protein 2 (c terminal) antibody
      • HSPABP2 antibody
      • NY CO 7 antibody
      • PP1131 antibody
      • SDCCAG7 antibody
      • Serologically defined colon cancer antigen 7 antibody
      • STIP1 homology and U Box containing protein 1 antibody
      • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
      • STIP1 homology and U box-containing protein 1 antibody
      • STUB 1 antibody
      • STUB1 antibody
      • UBOX 1 antibody
      • UBOX1 antibody
      see all

    Images

    • Western blot - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
      Western blot - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
      All lanes : Anti-STUB1/CHIP antibody [EPR4448] (ab109103) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : ECV304 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
      Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4448] (ab109103)

      Immunofluorescent staining of STUB1/CHIP in HeLa cells using ab109103 at 1/100 dilution.

    • Anti-STUB1/CHIP antibody [EPR4448] (ab109103)
      Anti-STUB1/CHIP antibody [EPR4448] (ab109103)

    References (3)

    Publishing research using ab109103? Please let us know so that we can cite the reference in this datasheet.

    ab109103 has been referenced in 3 publications.

    • Sugatani J  et al. Threonine-408 Regulates the Stability of Human Pregnane X Receptor through Its Phosphorylation and the CHIP/Chaperone-Autophagy Pathway. Drug Metab Dispos 44:137-50 (2016). PubMed: 26534988
    • El-Kasaby A  et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem 289:28987-9000 (2014). PubMed: 25202009
    • Bergmayr C  et al. Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. J Biol Chem 288:28831-44 (2013). PubMed: 23965991

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-STUB1 / CHIP antibody [EPR4448]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 10 2013

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