Anti-Substance P antibody [SP-DE4-21] (ab14184)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-240737-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Key features and details
- Mouse monoclonal [SP-DE4-21] to Substance P
- Suitable for: ELISA, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG1
Overview
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Product name
Anti-Substance P antibody [SP-DE4-21]
See all Substance P primary antibodies -
Description
Mouse monoclonal [SP-DE4-21] to Substance P -
Host species
Mouse -
Specificity
The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin). -
Tested applications
Suitable for: ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Human
Predicted to work with: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
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Immunogen
Synthetic peptide corresponding to Human Substance P aa 58-68.
Sequence:RPKPQQFFGLM
Database link: P20366 -
Positive control
- Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.75% Glycine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SP-DE4-21 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (12) |
1/1000 - 1/2000.
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
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IHC-P
1/1000 - 1/2000. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. -
Sequence similarities
Belongs to the tachykinin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 281512 Cow
- Entrez Gene: 6863 Human
- Entrez Gene: 21333 Mouse
- Entrez Gene: 24806 Rat
- Omim: 162320 Human
- SwissProt: P19850 Chicken
- SwissProt: P01289 Cow
- SwissProt: P67932 Guinea pig
see all -
Alternative names
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbs
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21716-anti-substance-p-antibody-sp-de4-21-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21715-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.com/ps/products/14/ab14184/Images/ab14184-21717-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab14184 has been referenced in 39 publications.
- Lu CC et al. The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat. Int J Mol Sci 23:N/A (2022). PubMed: 35216130
- Cheng Y et al. Network Pharmacology Analysis of Hewei Jiangni Granule for Gastroesophageal Reflux Disease and Experimental Verification of Its Anti-Neurogenic Inflammation Mechanism. Drug Des Devel Ther 16:1349-1363 (2022). PubMed: 35547866
- Nagashima T et al. Parabrachial-to-parasubthalamic nucleus pathway mediates fear-induced suppression of feeding in male mice. Nat Commun 13:7913 (2022). PubMed: 36585411
- Norton CE et al. Role of perivascular nerve and sensory neurotransmitter dysfunction in inflammatory bowel disease. Am J Physiol Heart Circ Physiol 320:H1887-H1902 (2021). PubMed: 33710922
- Kang DW et al. Bee venom reduces burn-induced pain via the suppression of peripheral and central substance P expression in mice. J Vet Sci 22:e9 (2021). PubMed: 33522161