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  1. Link

    products/primary-antibodies/substance-p-antibody-sp-de4-21-ab14184.pdf

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Neuroscience Neurotransmitter Neuropeptides Tachykinins
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Anti-Substance P antibody [SP-DE4-21] (ab14184)

  • Datasheet
  • SDS
Reviews (15)Q&A (17)References (34)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
  • Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)

Key features and details

  • Mouse monoclonal [SP-DE4-21] to Substance P
  • Suitable for: ELISA, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Guinea pig, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Substance P antibody [SP-DE4-21]
    See all Substance P primary antibodies
  • Description

    Mouse monoclonal [SP-DE4-21] to Substance P
  • Host species

    Mouse
  • Specificity

    The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
  • Tested applications

    Suitable for: ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human Substance P aa 58-68.
    Sequence:

    RPKPQQFFGLM


    Database link: P20366
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 0.75% Glycine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    SP-DE4-21
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Tachykinins
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Neurology process
    • Metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA (1)
Use at an assay dependent concentration.
IHC-P (12)
1/1000 - 1/2000.
ICC/IF (2)
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IHC-P
1/1000 - 1/2000.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
  • Sequence similarities

    Belongs to the tachykinin family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P20366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281512 Cow
    • Entrez Gene: 6863 Human
    • Entrez Gene: 21333 Mouse
    • Entrez Gene: 24806 Rat
    • Omim: 162320 Human
    • SwissProt: P19850 Chicken
    • SwissProt: P01289 Cow
    • SwissProt: P67932 Guinea pig
    • SwissProt: Q60541 Hamster
    • SwissProt: P67933 Horse
    • SwissProt: P20366 Human
    • SwissProt: P41539 Mouse
    • SwissProt: P06767 Rat
    • Unigene: 2563 Human
    • Unigene: 1440 Mouse
    • Unigene: 410823 Mouse
    • Unigene: 1920 Rat
    see all
  • Alternative names

    • C-terminal-flanking peptide antibody
    • Hs.2563 antibody
    • neurokinin 1 antibody
    • neurokinin 2 antibody
    • neurokinin A antibody
    • neurokinin alpha antibody
    • Neuromedin L antibody
    • neuropeptide gamma antibody
    • neuropeptide K antibody
    • NK2 antibody
    • NKA antibody
    • NKNA antibody
    • NPK antibody
    • PPT antibody
    • preprotachykinin antibody
    • protachykinin antibody
    • protachykinin-1 antibody
    • Substance K antibody
    • SubstanceP antibody
    • TAC1 antibody
    • TAC2 antibody
    • TAC2, formerly antibody
    • Tachykinin 1 antibody
    • tachykinin 2 antibody
    • tachykinin 2, formerly antibody
    • Tachykinin precursor 1 antibody
    • tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma) antibody
    • Tachykinin1 antibody
    • TKN1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbs

    ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.
    ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)
    Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.

Protocols

  • Recommended protocol for IHC-P with ab14184

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (34)

Publishing research using ab14184? Please let us know so that we can cite the reference in this datasheet.

ab14184 has been referenced in 34 publications.

  • Kang DW  et al. Bee venom reduces burn-induced pain via the suppression of peripheral and central substance P expression in mice. J Vet Sci 22:e9 (2021). PubMed: 33522161
  • Evdokimov D  et al. Characterization of dermal skin innervation in fibromyalgia syndrome. PLoS One 15:e0227674 (2020). PubMed: 31929578
  • Zhang Y  et al. Acupotomy Alleviates Energy Crisis at Rat Myofascial Trigger Points. Evid Based Complement Alternat Med 2020:5129562 (2020). PubMed: 32190087
  • He M  et al. Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus. Mol Med Rep 21:1163-1171 (2020). PubMed: 31922222
  • Rowe CW  et al. Innervation of papillary thyroid cancer and its association with extra-thyroidal invasion. Sci Rep 10:1539 (2020). PubMed: 32001748
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 32 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Substance P antibody [SP-DE4-21]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA and normal serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Rat Cell (Rat, free-floating brain slices, striatum and subs)
Specification
Rat, free-floating brain slices, striatum and subs
Permeabilization
Yes - Triton x-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Larisa Tratnjek

Verified customer

Submitted Mar 16 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain and jejunum)
Specification
Brain and jejunum
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 20 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cat Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 19 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Small intestine)
Specification
Small intestine
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 18 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Substance P antibody [SP-DE4-21]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Dog Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 18 2014

Question

Product # ab14184 substance P antibody I am having a difficult time with this antibody for immunohistochemistry. Fix samples in 4% paraformaldehyde overnight. 1. Paraffin sections 2. Citrate unmasking PBS wash 3. Block 2-5% goat serum in PBS-1hr room temp 4. 1:1000-1:500 SubP antibody 4*C overnight PBS wash 5. 1:100 goat anti-mouse Alexa 488 or Texas red-2hr room temp PBS wash Nothing but background seen on rabbit sciatic nerve, rat tendon, testis, skin, heart, muscle and lung. All negative controls (no primary) tissues, especially rabbit nerve, have fluorescent areas within the tissues. Have no indication that this antibody is working in any of my tissues.

Read More

Abcam community

Verified customer

Asked on Jun 19 2013

Answer

We have seen that enzymatic antigen retrieval is very successful when using this product on tissue in IHC-P and would recommend this. Have you attempted use of this type of antigen retrieval? I have added a link to Abcam's antigen retrieval guide for your review below. Antigen retrieval guide: https://www.abcam.com/index.html?pageconfig=resource&rid=11488

Read More

Keith B

Abcam Scientific Support

Answered on Jun 19 2013

1-10 of 32 Abreviews or Q&A

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