Recombinant Anti-SUCLA2 antibody [EPR14925] (ab183513)
![Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-215190-anti-sucla2-antibody-epr14925-western-blot.jpg "Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14925] to SUCLA2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SUCLA2 antibody [EPR14925]
See all SUCLA2 primary antibodies -
Description
Rabbit monoclonal [EPR14925] to SUCLA2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, 293, HepG2 and Molt4 cell lysates, Human breast tissue, HepG2 and 293 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14925 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
|
| IP |
1/40 - 1/60.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/40 - 1/60. |
Target
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Function
Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA. -
Tissue specificity
Widely expressed. Not expressed in liver and lung. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. -
Involvement in disease
Mitochondrial DNA depletion syndrome 5 -
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 8803 Human
- Entrez Gene: 20916 Mouse
- Entrez Gene: 361071 Rat
- Omim: 603921 Human
- SwissProt: Q9P2R7 Human
- SwissProt: Q9Z2I9 Mouse
- Unigene: 743361 Human
- Unigene: 38951 Mouse
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Alternative names
- A BETA antibody
- A SCS antibody
- ATP specific succinyl CoA synthetase subunit beta antibody
see all
Images
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Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa -
![Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-215196-anti-sucla2-antibody-epr14925-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (ab183513)Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.
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![Flow Cytometry (Intracellular) - Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-215193-anti-sucla2-antibody-epr14925-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SUCLA2 antibody [EPR14925] (ab183513)Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-215192-anti-sucla2-antibody-epr14925-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] (ab183513)Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-215191-anti-sucla2-antibody-epr14925-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] (ab183513)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SUCLA2 antibody [EPR14925] (ab183513)](https://www.abcam.com/ps/products/183/ab183513/Images/ab183513-6-benefits-of-recombinant-antibodies.png)
Anti-SUCLA2 antibody [EPR14925] (ab183513)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab183513 has been referenced in 1 publication.
- Wang W et al. Dl-3-n-butylphthalide attenuates mouse behavioral deficits to chronic social defeat stress by regulating energy metabolism via AKT/CREB signaling pathway. Transl Psychiatry 10:49 (2020). PubMed: 32066705
