Anti-Superoxide Dismutase 1 antibody (ab13498)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies -
Description
Rabbit polyclonal to Superoxide Dismutase 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk -
Immunogen
Synthetic peptide corresponding to Human Superoxide Dismutase 1.
Database link: P00441 -
Positive control
- WB: HeLa (Human epithelial cell line from cervix adenocarcinoma)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified on an antigen coupled sepharose column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| ELISA |
Use at an assay dependent concentration.
See Downs et al 2002. |
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (6) |
Use at an assay dependent concentration.
See Abreview. |
| IP |
Use at an assay dependent concentration.
See Shinder et al 2001. |
|
| WB | (13) |
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. See Downs et al 2002. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
IP
Use at an assay dependent concentration. See Shinder et al 2001. |
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WB
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localization
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 281495 Cow
- Entrez Gene: 39251 Drosophila melanogaster
- Entrez Gene: 6647 Human
- Entrez Gene: 20655 Mouse
- Entrez Gene: 397036 Pig
- Entrez Gene: 100009313 Rabbit
- Entrez Gene: 24786 Rat
- Entrez Gene: 100270717 Sheep
see all -
Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)Anti-Superoxide Dismutase 1 antibody (ab13498) at 0.2 µg/ml (2 hrs at RT) + HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP 1 hr at RT
Predicted band size: 16 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Block: 1.5% BSA.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (171)
ab13498 has been referenced in 171 publications.
- Linda K et al. Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders. Autophagy 18:423-442 (2022). PubMed: 34286667
- Dominiak K et al. Effects of Endurance Training on the Coenzyme Q Redox State in Rat Heart, Liver, and Brain at the Tissue and Mitochondrial Levels: Implications for Reactive Oxygen Species Formation and Respiratory Chain Remodeling. Int J Mol Sci 23:N/A (2022). PubMed: 35055078
- Zhu J et al. Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer. Oxid Med Cell Longev 2022:8038857 (2022). PubMed: 35087620
- Hu M et al. Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways. Bioengineered 13:3958-3968 (2022). PubMed: 35112986
- Jaroslawska J et al. Increasing energy expenditure through exercise and low ambient temperature offers oxidative protection to the hypothalamus after high-fat feeding to mice. J Neuroendocrinol 34:e13095 (2022). PubMed: 35138671