Name: Anti-Superoxide Dismutase 1 antibody [EP1727Y]
Brand: RabMAb
SKU: ab51254
Rating: 5 (3 reviews)

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Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Carrier Free HRP

Product name

Anti-Superoxide Dismutase 1 antibody [EP1727Y]
See all Superoxide Dismutase 1 primary antibodies
Description

Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.
Positive control
- IHC-P: Mouse cerebrum, rat liver and human breast carcinoma tissue WB: Jurkat cell lysate; Mouse and rat brain tissue lysates. ICC/IF: HeLa, PC-12 and NIH/3T3 cells. Flow Cyt (intra): HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1727Y
Isotype

IgG
Research areas
- Neuroscience
- Diseases

Alternative Versions
Assay kits
- Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
Recombinant Protein
- Recombinant human Superoxide Dismutase 1 protein (Active) (ab112193)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(2)	1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

Notes
Flow Cyt (Intra) 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Application notes

Is unsuitable for IP.

Function

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in disease

Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Sequence similarities

Belongs to the Cu-Zn superoxide dismutase family.
Post-translational
modifications

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Cellular localization

Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
Target information above from: UniProt accession P00441 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6647 Human
- Entrez Gene: 20655 Mouse
- Entrez Gene: 24786 Rat
- Omim: 147450 Human
- SwissProt: P00441 Human
- SwissProt: P08228 Mouse
- SwissProt: P07632 Rat
- Unigene: 443914 Human
- Unigene: 276325 Mouse
- Unigene: 466779 Mouse
- Unigene: 6059 Rat
see all
Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
- Cu/Zn SOD antibody
- Cu/Zn superoxide dismutase antibody
- Epididymis secretory protein Li 44 antibody
- HEL S 44 antibody
- Homodimer antibody
- hSod1 antibody
- Indophenoloxidase A antibody
- IPOA antibody
- Mn superoxide dismutase antibody
- SOD antibody
- SOD soluble antibody
- SOD1 antibody
- SOD2 antibody
- SODC antibody
- SODC_HUMAN antibody
- Superoxide dismutase [Cu-Zn] antibody
- Superoxide dismutase 1 antibody
- Superoxide dismutase 1 soluble antibody
- Superoxide dismutase Cu Zn antibody
- Superoxide dismutase cystolic antibody
see all

Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

ab51254 was shown to react with SOD1 in wild-type HeLa cells in Western blot with loss of signal observed in a SOD1 knockout cell line. Wild-type HeLa and SOD1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab51254 overnight at 4 °C at a 1/15000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 2 ug/ml dilution.
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

All lanes : Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 0.015 µg/ml

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Blocking/diluting Buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Flow Cytometry (Intracellular) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Superoxide Dismutase 1 with ab51254 at 1/20 dilution (1µg) (Red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent staining of HeLa cells using ab51254 (1:100).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab51254? Please let us know so that we can cite the reference in this datasheet.

ab51254 has been referenced in 31 publications.

Hu M et al. Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways. Bioengineered 13:3958-3968 (2022). PubMed: 35112986
Delport A & Hewer R A superior loading control for the cellular thermal shift assay. Sci Rep 12:6672 (2022). PubMed: 35461337
Costanzo-Garvey DL et al. Prostate cancer addiction to oxidative stress defines sensitivity to anti-tumor neutrophils. Clin Exp Metastasis 39:641-659 (2022). PubMed: 35604506
Shin CY et al. Protective Effect of Ribes nigrum Extract against Blue Light-Induced Retinal Degeneration In Vitro and In Vivo. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624696
Liu Q et al. Inhibition of TRPA1 Ameliorates Periodontitis by Reducing Periodontal Ligament Cell Oxidative Stress and Apoptosis via PERK/eIF2α/ATF-4/CHOP Signal Pathway. Oxid Med Cell Longev 2022:4107915 (2022). PubMed: 35720191

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application

Western blot

Sample

Mouse Tissue lysate - whole (Balb/C mice brain)

Gel Running Conditions

Reduced Denaturing (4-20% tris-glycine gel)

Loading amount

40 µg

Specification

Balb/C mice brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 15 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (SHSY5Y cells)

Gel Running Conditions

Reduced Denaturing (4-20% tris-glycin gel)

Loading amount

40 µg

Specification

SHSY5Y cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 14 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Pig Tissue sections (Ovary)

Permeabilization

Specification

Ovary

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Natasja Costermans

Verified customer

Submitted Aug 07 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (31)

Customer reviews and Q&As

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Western blot abreview for Anti-Superoxide Dismutase 1 antibody [EP1727Y]

Western blot abreview for Anti-Superoxide Dismutase 1 antibody [EP1727Y]

Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody [EP1727Y]

Post-translational
modifications