Recombinant Anti-Superoxide Dismutase 1 antibody [EPR20577-5] - BSA and Azide free (Detector) (ab285139)
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Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20577-5] to Superoxide Dismutase 1 - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody [EPR20577-5] - BSA and Azide free (Detector)
See all Superoxide Dismutase 1 primary antibodies -
Description
Rabbit monoclonal [EPR20577-5] to Superoxide Dismutase 1 - BSA and Azide free (Detector) -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
General notes
ab285139 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Superoxide Dismutase 1. The recommended pair for sandwich ELISA is:
Capture: ab285033, Human Superoxide Dismutase 1 Capture Antibody (unconjugated)
Detector: ab285139, Human Superoxide Dismutase 1 Detector Antibody (unconjugated)
The reference range value is 0.39 - 50 ng/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20577-5 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
- Anti-Superoxide Dismutase 1 antibody [EPR20577-29] - BSA and Azide free (Capture) (ab285033)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab285139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR20577-29] to Superoxide Dismutase 1 - BSA and Azide free (Capture) (ab285033).
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR20577-29] to Superoxide Dismutase 1 - BSA and Azide free (Capture) (ab285033). |
Target
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Function
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family. -
Post-translational
modificationsUnlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
Cellular localization
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00441 Human
- Unigene: 443914 Human
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Alternative names
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
Images
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Sandwich ELISA - Anti-Superoxide Dismutase 1 antibody [EPR20577-5] - BSA and Azide free (Detector) (ab285139)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab285139 has not yet been referenced specifically in any publications.
