Recombinant Anti-Swd2 antibody [EPR27034-63] (ab307554)

Product name

Anti-Swd2 antibody [EPR27034-63]
See all Swd2 primary antibodies
Description

Rabbit monoclonal [EPR27034-63] to Swd2
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HeLa transfected with siRNA specifically targeting Swd2, HeLa, HCT116, HepG2, NIH/3T3, Human colon, Mouse colon and Rat colon lysates. IHC-P: Human colon, Human colon cancer, Mouse colon and Rat colon tissues. ICC/IF: HeLa and NIH/3T3 cells Flow Cyt (intra): HeLa and NIH/3T3 cells IP: Human colon and Mouse colon cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR27034-63
Isotype

IgG
Research areas

Alternative Versions
- Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/500.
WB		1/1000. Predicted molecular weight: 35 kDa.
IHC-P		1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/50.
IP		1/30.

Notes
Flow Cyt (Intra) 1/500.
WB 1/1000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
IP 1/30.

Function

Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similarities

Belongs to the WD repeat SWD2 family.
Contains 6 WD repeats.
Cellular localization

Nucleus. Associates with chromatin.
Target information above from: UniProt accession Q6UXN9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 80335 Human
- Entrez Gene: 77305 Mouse
- Entrez Gene: 686295 Rat
- Omim: 611059 Human
- SwissProt: Q6UXN9 Human
- SwissProt: Q8BFQ4 Mouse
- Unigene: 194110 Human
Alternative names
- TMEM113 antibody
- MST107 antibody
- MSTP107 antibody
- PRO2730 antibody
- PRO34047 antibody
- Protein TMEM113 antibody
- Swd2 antibody
- transmembrane protein 113 antibody
- UNQ9342/PRO34047 antibody
- WD repeat domain 82 antibody
- WD repeat-containing protein 82 antibody
- wdr82 antibody
- WDR82_HUMAN antibody
- WDR82A antibody
see all

Western blot - Anti-Swd2 antibody [EPR27034-63] (ab307554)

All lanes : Anti-Swd2 antibody [EPR27034-63] (ab307554) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting Swd2, whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 27 seconds
Western blot - Anti-Swd2 antibody [EPR27034-63] (ab307554)

All lanes : Anti-Swd2 antibody [EPR27034-63] (ab307554) at 1/1000 dilution

Lane 1 : Human colon tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : Rat colon tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 103 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Swd2 with ab307554 at 1/500 (1.1 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon (PMID: 30008910). The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Swd2 with ab307554 at 1/500 (1.1 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon cancer (PMID: 30008910). The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Swd2 with ab307554 at 1/2000 (0.275 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse colon. The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Swd2 with ab307554 at 1/2000 (0.275 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Swd2 with ab307554 at 1/50 (11.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Swd2 with ab307554 at 1/50 (11.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Swd2 with ab307554 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Swd2 with ab307554 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Swd2 was immunoprecipitated from 0.35 mg human colon tissue lysate 10 ug with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: human colon tissue lysate 10 ug

Lane 2: abab307554 IP in Mouse colon tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307554 in human colon tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 67 seconds
Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] (ab307554)

Swd2 was immunoprecipitated from 0.35 mg Mouse colon tissue lysate 10 ug with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse colon tissue lysate 10 ug

Lane 2: abab307554 IP in Mouse colon tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307554 in mouse colon tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 67 seconds
Anti-Swd2 antibody [EPR27034-63] (ab307554)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As