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  1. Link

    products/primary-antibodies/synaptogyrin-3-antibody-ab113712.pdf

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Neuroscience Neurotransmission Secretory Vesicles Regulation
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Anti-Synaptogyrin 3 antibody (ab113712)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody (ab113712)
  • Western blot - Anti-Synaptogyrin 3 antibody (ab113712)

Key features and details

  • Rabbit polyclonal to Synaptogyrin 3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Synaptogyrin 3 antibody
    See all Synaptogyrin 3 primary antibodies
  • Description

    Rabbit polyclonal to Synaptogyrin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from within C terminal region of Human Synaptogyrin 3, conjugated to KLH.

  • Positive control

    • Human Placenta tissue. Mouse brain tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Regulation
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (0 days) (ab7188)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the positive regulation of dopamine transporter activity. Probably facilitates the physical and functional interactions of the transporter with the dopamine vesicular storage system, allowing a more efficient loading of the vesicles with extracellular dopamine after release.
  • Tissue specificity

    Expressed in brain and placenta.
  • Sequence similarities

    Belongs to the synaptogyrin family.
    Contains 1 MARVEL domain.
  • Cellular localization

    Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. Cell junction > synapse. Found at the neuromuscular synapses.
  • Target information above from: UniProt accession O43761 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9143 Human
    • Entrez Gene: 20974 Mouse
    • Entrez Gene: 302975 Rat
    • Omim: 603927 Human
    • SwissProt: O43761 Human
    • SwissProt: Q8R191 Mouse
    • Unigene: 435277 Human
    • Unigene: 26032 Mouse
    • Alternative names

      • MGC:20003 antibody
      • MGC20003 antibody
      • OTTHUMP00000158899 antibody
      • SNG3_HUMAN antibody
      • Synaptogyrin 3 antibody
      • Synaptogyrin-3 antibody
      • Synaptogyrin3 antibody
      • SYNGR 3 antibody
      • SYNGR3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody (ab113712)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptogyrin 3 antibody (ab113712)

      ab113712 at 5 µg/ml staining Synaptogyrin 3 in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.

    • Western blot - Anti-Synaptogyrin 3 antibody (ab113712)
      Western blot - Anti-Synaptogyrin 3 antibody (ab113712)
      Lane 1 : Anti-Synaptogyrin 3 antibody (ab113712) at 1 µg/ml
      Lane 2 : Anti-Synaptogyrin 3 antibody (ab113712) at 2 µg/ml

      All lanes : Mouse brain tissue lysate

      Predicted band size: 25 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab113712? Please let us know so that we can cite the reference in this datasheet.

    ab113712 has not yet been referenced specifically in any publications.

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