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RecombinantRabMAb

Recombinant Anti-SYPL1 antibody [EPR14317] (ab184176)

Submit a review Submit a question References (5)
Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
  • Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
  • Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] (ab184176)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
  • Anti-SYPL1 antibody [EPR14317] (ab184176)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14317] to SYPL1
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SYPL1 antibody [EPR14317]
    See all SYPL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14317] to SYPL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, fetal lung, fetal kidney and adipose tissue lysates; Human ovarian carcinoma and colon tissues; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14317

  • Isotype

    IgG

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa).
Flow Cyt (Intra)
1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa).
Flow Cyt (Intra)
1/130.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • Cellular localization

    Cytoplasmic vesicle membrane. Melanosome. Cytoplasmic transport vesicles (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • H-SP1 antibody
      • Pantophysin antibody
      • Synaptophysin-like protein 1 antibody
      • SYPL antibody
      • Sypl1 antibody
      • SYPL1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
      Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
      All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/5000 dilution

      Lane 1 : Human fetal lung tissue lysate
      Lane 2 : Human fetal liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 29 kDa

    • Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
      Western blot - Anti-SYPL1 antibody [EPR14317] (ab184176)
      All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human adipose tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 29 kDa

    • Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] (ab184176)
      Flow Cytometry (Intracellular) - Anti-SYPL1 antibody [EPR14317] (ab184176)

      Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%)labeling SYPL1 with ab184176 at 1/130 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-SYPL1 antibody [EPR14317] (ab184176)
      Anti-SYPL1 antibody [EPR14317] (ab184176)

    References (5)

    Publishing research using ab184176? Please let us know so that we can cite the reference in this datasheet.

    ab184176 has been referenced in 5 publications.

    • Situ J  et al. Comparative Proteomic Analysis Reveals the Effect of Selenoprotein W Deficiency on Oligodendrogenesis in Fear Memory. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624863
    • Zhao L  et al. Effects of Fibroblast Growth Factor 21 on Lactate Uptake and Usage in Mice with Diabetes-Associated Cognitive Decline. Mol Neurobiol 59:5656-5672 (2022). PubMed: 35761156
    • Liu L  et al. Serum SYPL1 is a promising diagnostic biomarker for colorectal cancer. Clin Chim Acta 509:36-42 (2020). PubMed: 32502495
    • Song Y  et al. SYPL1 Inhibits Apoptosis in Pancreatic Ductal Adenocarcinoma via Suppression of ROS-Induced ERK Activation. Front Oncol 10:1482 (2020). PubMed: 33042794
    • Chen DH  et al. SYPL1 overexpression predicts poor prognosis of hepatocellular carcinoma and associates with epithelial-mesenchymal transition. Oncol Rep 38:1533-1542 (2017). PubMed: 28731154

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