Recombinant Anti-SYPL1 antibody [EPR14317] (ab184176)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14317] to SYPL1
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SYPL1 antibody [EPR14317]
See all SYPL1 primary antibodies -
Description
Rabbit monoclonal [EPR14317] to SYPL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, fetal lung, fetal kidney and adipose tissue lysates; Human ovarian carcinoma and colon tissues; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14317 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa).
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Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 29-37 kDa (predicted molecular weight: 29 kDa). |
Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
Cellular localization
Cytoplasmic vesicle membrane. Melanosome. Cytoplasmic transport vesicles (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6856 Human
- SwissProt: Q16563 Human
- Unigene: 80919 Human
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Alternative names
- H-SP1 antibody
- Pantophysin antibody
- Synaptophysin-like protein 1 antibody
see all
Images
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All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/5000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa -
All lanes : Anti-SYPL1 antibody [EPR14317] (ab184176) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human adipose tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa -
Intracellular flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%)labeling SYPL1 with ab184176 at 1/130 dilution (red) or a rabbit IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYPL1 antibody [EPR14317] (ab184176)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SYPL1 with ab184176 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab184176 has been referenced in 5 publications.
- Situ J et al. Comparative Proteomic Analysis Reveals the Effect of Selenoprotein W Deficiency on Oligodendrogenesis in Fear Memory. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624863
- Zhao L et al. Effects of Fibroblast Growth Factor 21 on Lactate Uptake and Usage in Mice with Diabetes-Associated Cognitive Decline. Mol Neurobiol 59:5656-5672 (2022). PubMed: 35761156
- Liu L et al. Serum SYPL1 is a promising diagnostic biomarker for colorectal cancer. Clin Chim Acta 509:36-42 (2020). PubMed: 32502495
- Song Y et al. SYPL1 Inhibits Apoptosis in Pancreatic Ductal Adenocarcinoma via Suppression of ROS-Induced ERK Activation. Front Oncol 10:1482 (2020). PubMed: 33042794
- Chen DH et al. SYPL1 overexpression predicts poor prognosis of hepatocellular carcinoma and associates with epithelial-mesenchymal transition. Oncol Rep 38:1533-1542 (2017). PubMed: 28731154