Anti-SYT2 antibody [ZNP-1] (ab154035)
![Western blot - Anti-SYT2 antibody [ZNP-1] (ab154035)](https://www.abcam.com/ps/products/154/ab154035/Images/ab154035-159842-anti-syt2-antibody-znp-1-western-blot.jpg "Western blot - Anti-SYT2 antibody [ZNP-1] (ab154035)")
Key features and details
- Mouse monoclonal [ZNP-1] to SYT2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG2a
Overview
-
Product name
Anti-SYT2 antibody [ZNP-1]
See all SYT2 primary antibodies -
Description
Mouse monoclonal [ZNP-1] to SYT2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Does not react with: Cow -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- Human, Rat, Mouse and Zebrafish Brain Homogenate Lysate; SH-SY5Y cells. IHC-P: Mouse and rat cerebellum tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: Human serum albumin -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab154035 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. -
Clonality
Monoclonal -
Clone number
ZNP-1 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
-
Database links
- Entrez Gene: 127833 Human
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Omim: 600104 Human
- SwissProt: Q8N9I0 Human
- SwissProt: P46097 Mouse
- SwissProt: P29101 Rat
- Unigene: 25422 Human
see all -
Alternative names
- Synaptotagmin 2 antibody
- Synaptotagmin II antibody
- Synaptotagmin-2 antibody
see all
Images
-
Western blot - Anti-SYT2 antibody [ZNP-1] (ab154035)All lanes : Anti-SYT2 antibody [ZNP-1] (ab154035) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Brain Homogenate Lysate
Lane 3 : Mouse Brain Homogenate Lysate
Lane 4 : Zebrafish Brain Homogenate Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG – HRP at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody [ZNP-1] (ab154035)](https://www.abcam.com/ps/products/154/ab154035/Images/ab154035-4-anti-syt2-antibody-znp1-immunohistochemistry-cerebellum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody [ZNP-1] (ab154035)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue sections labeling SYT2 with purified ab154035 at 1/4000 dilution (0.25 µg/mL). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes. Leica mouse (DS9800) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody [ZNP-1] (ab154035)](https://www.abcam.com/ps/products/154/ab154035/Images/ab154035-3-anti-syt2-antibody-znp1-immunohistochemistry-cerebellum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody [ZNP-1] (ab154035)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue sections labeling SYT2 with purified ab154035 at 1/4000 dilution (0.25 µg/mL). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes. Leica mouse (DS9800) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain. -
![Immunocytochemistry/ Immunofluorescence - Anti-SYT2 antibody [ZNP-1] (ab154035)](https://www.abcam.com/ps/products/154/ab154035/Images/ab154035-159841-anti-syt2-antibody-znp-1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SYT2 antibody [ZNP-1] (ab154035)Immunocytochemistry with SH-SY5Y cells stained with Anti-SYT2 antibody (ab154035) in red and DAPI in blue, as a counter stain. The target protein locates to the plasma membrane (red).Fixation: 4% Paraformaldehyde, 20 min.
Permeabilization: 0.1% Triton X-100, 10min.
Blocking: 10% Goat Serum/PBS, 1 hour.
Primary: Anti-SYT2 antibody (ab154035) 5 µg/ml in 10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C.
Secondary: Alexa Fluor® 594 Goat Anti-Mouse (H&L) used at a 1/1000 dilution in 10% Goat Serum/PBS, 1hr at RT.
Washing: 3x 1% Goat Serum/PBS, 10 mins/wash
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154035 has not yet been referenced specifically in any publications.