Recombinant Anti-TAGAP antibody [EPR15593] (ab187664)
![Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] (ab187664)](https://www.abcam.com/ps/products/187/ab187664/Images/ab187664-220667-anti-tagap-antibody-epr15593-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] (ab187664)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15593] to TAGAP
- Suitable for: IP, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TAGAP antibody [EPR15593]
See all TAGAP primary antibodies -
Description
Rabbit monoclonal [EPR15593] to TAGAP -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- A549, Jurkat and HepG2 whole cell lysate (ab7900); Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15593 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/20 - 1/40.
|
|
| Flow Cyt (Intra) |
1/160.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/2000. Detects a band of approximately 61, 81 kDa (predicted molecular weight: 81 kDa).
|
| Notes |
|---|
|
IP
1/20 - 1/40. |
|
Flow Cyt (Intra)
1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 61, 81 kDa (predicted molecular weight: 81 kDa). |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 117289 Human
- GenBank: NM_054114.3 Human
- Omim: 609667 Human
- SwissProt: Q8N103 Human
- Unigene: 529984 Human
-
Alternative names
- 2610315E15Rik antibody
- ARHGAP47 antibody
- FKSG15 antibody
see all
Images
-
Flow Cytometry (Intracellular) - Anti-TAGAP antibody [EPR15593] (ab187664)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat cells labeling TAGAP with ab187664 at 1/160 dilution (pink) compared to aRabbit monoclonal IgG Isotype control (green). Goat anti rabbit IgG (FITC) was used as the secondary antibody at 1/150 dilution.
-
![Western blot - Anti-TAGAP antibody [EPR15593] (ab187664)](https://www.abcam.com/ps/products/187/ab187664/Images/ab187664-220668-anti-tagap-antibody-epr15593-western-blot.jpg)
Western blot - Anti-TAGAP antibody [EPR15593] (ab187664)All lanes : Anti-TAGAP antibody [EPR15593] (ab187664) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 61,81 kDa why is the actual band size different from the predicted?Based on sequence analysis, ab187664 recognizes three isoforms with the predicted MWs of 81kDa, 61kDa and 57kDa, respectively.
-
![Immunoprecipitation - Anti-TAGAP antibody [EPR15593] (ab187664)](https://www.abcam.com/ps/products/187/ab187664/Images/ab187664-220666-anti-tagap-antibody-epr15593-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TAGAP antibody [EPR15593] (ab187664)Western blot analysis of TAGAP in A549 cell lysate immunoprecipitated with ab187664 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
-
![Anti-TAGAP antibody [EPR15593] (ab187664)](https://www.abcam.com/ps/products/187/ab187664/Images/ab187664-4-benefits-of-recombinant-antibodies.png)
Anti-TAGAP antibody [EPR15593] (ab187664)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab187664 has been referenced in 3 publications.
- Chen J et al. TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response. Nat Commun 11:1913 (2020). PubMed: 32312989
- McGuinness D et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). PubMed: 30094915
- Berge T et al. The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells. Genes Immun 17:118-27 (2016). Flow Cyt ; Human . PubMed: 26765264