Recombinant Anti-TARBP1 antibody [EPR8727] (ab150412)
![Western blot - Anti-TARBP1 antibody [EPR8727] (ab150412)](https://www.abcam.com/ps/products/150/ab150412/Images/ab150412-157053-anti-tarbp1-antibody-epr8727-western-blot.jpg "Western blot - Anti-TARBP1 antibody [EPR8727] (ab150412)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8727] to TARBP1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TARBP1 antibody [EPR8727]
See all TARBP1 primary antibodies -
Description
Rabbit monoclonal [EPR8727] to TARBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TARBP1 aa 200-300. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, HepG2, 293T and Caco 2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8727 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 182 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 182 kDa. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Probable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA. -
Sequence similarities
Belongs to the RNA methyltransferase TrmH family. - Information by UniProt
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Database links
- Entrez Gene: 6894 Human
- Omim: 605052 Human
- SwissProt: Q13395 Human
- Unigene: 498115 Human
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Alternative names
- FLJ30482 antibody
- Probable methyltransferase TARBP1 antibody
- TAR (HIV) RNA binding protein 1 antibody
see all
Images
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Western blot - Anti-TARBP1 antibody [EPR8727] (ab150412)All lanes : Anti-TARBP1 antibody [EPR8727] (ab150412) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 182 kDa -
![Anti-TARBP1 antibody [EPR8727] (ab150412)](https://www.abcam.com/ps/products/150/ab150412/Images/ab150412-2-benefits-of-recombinant-antibodies.png)
Anti-TARBP1 antibody [EPR8727] (ab150412)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150412 has been referenced in 1 publication.
- Freund I et al. 2'-O-methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms. RNA 25:869-880 (2019). PubMed: 31019095