Recombinant Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228741-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
See all Tartrate Resistant Acid Phosphatase primary antibodies -
Description
Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase -
Host species
Rabbit -
Specificity
This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions. -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431, MDA-MB-435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB-435 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15556 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/50.
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| ICC/IF |
1/100.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa).
Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform. |
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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IP
1/50. |
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ICC/IF
1/100. |
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WB
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa). Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. -
Sequence similarities
Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 54 Human
- Entrez Gene: 11433 Mouse
- Entrez Gene: 25732 Rat
- Omim: 171640 Human
- SwissProt: P13686 Human
- SwissProt: Q05117 Mouse
- SwissProt: P29288 Rat
- Unigene: 1211 Human
see all -
Alternative names
- Type 5 acid phosphatase antibody
- Acid phosphatase 5 tartrate resistant antibody
- Acid phosphatase 5, tartrate resistant antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228744-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-western-blot.jpg)
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228743-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-western-blot.jpg)
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 35,42 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.
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![Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228742-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-western-blot.jpg)
Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM /TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228740-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).
The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
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![Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-228768-anti-tartrate-resistant-acid-phosphatase-antibody-epr15556-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.
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![Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)](https://www.abcam.com/ps/products/191/ab191406/Images/ab191406-7-benefits-of-recombinant-antibodies.png)
Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (18)
ab191406 has been referenced in 18 publications.
- Zhang W et al. Rosavin suppresses osteoclastogenesis in vivo and in vitro by blocking the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) and mitogen-activated protein kinase (MAPK) signaling pathways. Ann Transl Med 9:383 (2021). PubMed: 33842604
- Jiang C et al. Oxymatrine Attenuates Osteoclastogenesis via Modulation of ROS-Mediated SREBP2 Signaling and Counteracts Ovariectomy-Induced Osteoporosis. Front Cell Dev Biol 9:684007 (2021). PubMed: 34136493
- Wang R et al. Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis. J Int Med Res 48:300060520978015 (2020). PubMed: 33322976
- Li G et al. Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion. Cell Cycle 19:1022-1035 (2020). PubMed: 32208888
- Li P et al. Loss of PARP-1 attenuates diabetic arteriosclerotic calcification via Stat1/Runx2 axis. Cell Death Dis 11:22 (2020). PubMed: 31924749