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    products/primary-antibodies/tartrate-resistant-acid-phosphatase-antibody-epr15556-ab191406.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (18)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
  • Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Alexa Fluor® 488 Carrier Free

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Overview

  • Product name

    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
    See all Tartrate Resistant Acid Phosphatase primary antibodies
  • Description

    Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
  • Host species

    Rabbit
  • Specificity

    This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions.
  • Tested applications

    Suitable for: IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, MDA-MB-435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB-435 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15556
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
    • Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] - BSA and Azide free (ab240970)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human Tartrate Resistant Acid Phosphatase protein (Active) (ab222972)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50.
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa).

Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IP
1/50.
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa).

Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
  • Sequence similarities

    Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession P13686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54 Human
    • Entrez Gene: 11433 Mouse
    • Entrez Gene: 25732 Rat
    • Omim: 171640 Human
    • SwissProt: P13686 Human
    • SwissProt: Q05117 Mouse
    • SwissProt: P29288 Rat
    • Unigene: 1211 Human
    • Unigene: 46354 Mouse
    • Unigene: 171928 Rat
    see all
  • Alternative names

    • Type 5 acid phosphatase antibody
    • Acid phosphatase 5 tartrate resistant antibody
    • Acid phosphatase 5, tartrate resistant antibody
    • ACP5 antibody
    • EC 3.1.3.2 antibody
    • MGC117378 antibody
    • PPA5_HUMAN antibody
    • serum band 5 tartrate-resistant acid phosphatase antibody
    • SPENCDI antibody
    • T5ap antibody
    • Tartrate resistant acid ATPase antibody
    • Tartrate resistant acid phosphatase type 5 antibody
    • Tartrate resistant acid phosphatase type 5 precursor antibody
    • Tartrate-resistant acid ATPase antibody
    • Tartrate-resistant acid phosphatase type 5 antibody
    • TR AP antibody
    • TR-AP antibody
    • TRACP 5 antibody
    • TRAP antibody
    • TrATPase antibody
    • Type 5 acid phosphatase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35,42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

     

    Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.

  • Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized MDA-MB-435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Immunoprecipitation - Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

    Western blot analysis of TRAP 5 in MDA-MB-435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.

  • Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)
    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (18)

Publishing research using ab191406? Please let us know so that we can cite the reference in this datasheet.

ab191406 has been referenced in 18 publications.

  • Zhang W  et al. Rosavin suppresses osteoclastogenesis in vivo and in vitro by blocking the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) and mitogen-activated protein kinase (MAPK) signaling pathways. Ann Transl Med 9:383 (2021). PubMed: 33842604
  • Jiang C  et al. Oxymatrine Attenuates Osteoclastogenesis via Modulation of ROS-Mediated SREBP2 Signaling and Counteracts Ovariectomy-Induced Osteoporosis. Front Cell Dev Biol 9:684007 (2021). PubMed: 34136493
  • Wang R  et al. Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis. J Int Med Res 48:300060520978015 (2020). PubMed: 33322976
  • Li G  et al. Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion. Cell Cycle 19:1022-1035 (2020). PubMed: 32208888
  • Li P  et al. Loss of PARP-1 attenuates diabetic arteriosclerotic calcification via Stat1/Runx2 axis. Cell Death Dis 11:22 (2020). PubMed: 31924749
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (vertebrae)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
vertebrae
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
10%NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Zhirui Jiang

Verified customer

Submitted Mar 13 2019

IHC - Wholemount abreview for Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Whole Tibia)
Specification
Whole Tibia
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 24 2018

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