Recombinant Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)
![Western blot - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-1.JPG "Western blot - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9104(B)] to Tat-SF1
- Suitable for: WB, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tat-SF1 antibody [EPR9104(B)]
See all Tat-SF1 primary antibodies -
Description
Rabbit monoclonal [EPR9104(B)] to Tat-SF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Tat-SF1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat and HeLa cell lysates; Human colon and uterus tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9104(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 86 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| ICC/IF |
1/250 - 1/500.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 86 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
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ICC/IF
1/250 - 1/500. |
Target
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Relevance
HIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 27336 Human
- Entrez Gene: 72459 Mouse
- Omim: 300346 Human
- SwissProt: O43719 Human
- SwissProt: Q8BGC0 Mouse
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Alternative names
- Cofactor required for Tat activation of HIV 1 transcription antibody
- dJ196E23.2 antibody
- HIV Tat specific factor 1 antibody
see all
Images
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Western blot - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)All lanes : Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/HIV-TAT-specific-factor-1-Primary-antibodies-ab140654-4.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)Immunofluorescent analysis of HeLa cells labelling Tat-SF1 with ab140654 at 1/250 dilution.
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![Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)](https://www.abcam.com/ps/products/140/ab140654/Images/ab140654-5-benefits-of-recombinant-antibodies.png)
Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab140654 has not yet been referenced specifically in any publications.