Recombinant Anti-TBR1 antibody [EPR8138(2)] (ab183032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8138(2)] to TBR1
- Suitable for: ICC/IF, WB, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TBR1 antibody [EPR8138(2)]
See all TBR1 primary antibodies -
Description
Rabbit monoclonal [EPR8138(2)] to TBR1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate. ICC/IF: Mouse Neuron Cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8138(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
1/100.
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WB | (1) |
1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
IHC-Fr |
1/100.
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IHC-P |
1/80000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We don’t recommend this antibody for IHC in human. Under our experimental conditions, this antibody showed no positive staining on human fetal cerebral cortex and weak cytoplasmic staining on human glioma. |
Notes |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
IHC-Fr
1/100. |
IHC-P
1/80000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We don’t recommend this antibody for IHC in human. Under our experimental conditions, this antibody showed no positive staining on human fetal cerebral cortex and weak cytoplasmic staining on human glioma. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 10716 Human
- Entrez Gene: 21375 Mouse
- Entrez Gene: 680427 Rat
- Omim: 604616 Human
- SwissProt: Q16650 Human
- SwissProt: Q64336 Mouse
- Unigene: 210862 Human
- Unigene: 308525 Mouse
see all -
Alternative names
- T box brain 1 antibody
- T box brain protein 1 antibody
- T brain 1 protein antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2µg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).
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ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].
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Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].
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Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/ml + Human brain lysate at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Predicted band size: 74 kDa
Exposure time: 30 secondsThe blocking and dilution buffer used was 5% NFDM/TBST
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ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].
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Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml. Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].
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Ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1:300 dilution) at 4oC for 12 hours. An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.
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All lanes : Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/ml
Lane 1 : Mouse P1 Cerebral cortex lysate.
Lane 2 : Mouse hippocampus lysate
Lane 3 : E18 rat brain lysate
Lane 4 : Rat P5 cerebral cortex lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 74 kDaThe blocking and dilution buffer used was 5% NFDM/TBST.
Exposure time
Lane 1 and 2: 10 seconds;
Lane 3: 5 seconds;
Lane 4: 2 seconds;
Lane 5: 3 minutes;
Lane 6: 15 seconds -
Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 1/10000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 74 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (14)
ab183032 has been referenced in 14 publications.
- Iwasawa E et al. The Anti-Inflammatory Agent Bindarit Attenuates the Impairment of Neural Development through Suppression of Microglial Activation in a Neonatal Hydrocephalus Mouse Model. J Neurosci 42:1820-1844 (2022). PubMed: 34992132
- Zhou YP et al. A congenital CMV infection model for follow-up studies of neurodevelopmental disorders, neuroimaging abnormalities, and treatment. JCI Insight 7:N/A (2022). PubMed: 35014624
- Schrötter S et al. The non-essential TSC complex component TBC1D7 restricts tissue mTORC1 signaling and brain and neuron growth. Cell Rep 39:110824 (2022). PubMed: 35584673
- Motosugi N et al. De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs. Cell Rep Methods 2:100352 (2022). PubMed: 36590687
- Guttikonda SR et al. Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer's disease. Nat Neurosci 24:343-354 (2021). PubMed: 33558694