Recombinant Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266946-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19012] to TBR2 / Eomes
- Suitable for: ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-TBR2 / Eomes antibody [EPR19012]
See all TBR2 / Eomes primary antibodies -
Description
Rabbit monoclonal [EPR19012] to TBR2 / Eomes -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC/IF: Mouse primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19012 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/200.
|
|
| IHC-Fr | (3) |
1/1000.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/200. |
|
IHC-Fr
1/1000. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. -
Tissue specificity
Expressed in CD8+ T-cells. -
Involvement in disease
Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Developmental stage
Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 13813 Mouse
- SwissProt: O54839 Mouse
- Unigene: 200692 Mouse
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Alternative names
- eomes antibody
- EOMES_HUMAN antibody
- Eomesodermin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-7-anti-tbr2eomes-antibody-epr19012-immunocytochemistry-mouse-primary-neuron-cell1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266944-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on E14.5 mouse cortex is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266945-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266943-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Specific staining on adult mouse hippocampus is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-266942-anti-tbr2-eomes-antibody-epr19012-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Negative control tissue; very weak staining is due to autoflorescence of the kidney section.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)](https://www.abcam.com/ps/products/183/ab183991/Images/ab183991-8-benefits-of-recombinant-antibodies.png)
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (26)
ab183991 has been referenced in 26 publications.
- Ezan J et al. Early loss of Scribble affects cortical development, interhemispheric connectivity and psychomotor activity. Sci Rep 11:9106 (2021). PubMed: 33907211
- Harlan De Crescenzo A et al. Deficient or Excess Folic Acid Supply During Pregnancy Alter Cortical Neurodevelopment in Mouse Offspring. Cereb Cortex 31:635-649 (2021). PubMed: 32995858
- Morandell J et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nat Commun 12:3058 (2021). PubMed: 34031387
- Ichinose M et al. The BMP antagonist gremlin 1 contributes to the development of cortical excitatory neurons, motor balance and fear responses. Development 148:N/A (2021). PubMed: 34184027
- Johnston S et al. AAV ablates neurogenesis in the adult murine hippocampus. Elife 10:N/A (2021). PubMed: 34259630