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    products/primary-antibodies/tbr2--eomes-antibody-epr19012-ab183991.pdf

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RecombinantRabMAb

Recombinant Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (7) Submit a question References (26)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
  • Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19012] to TBR2 / Eomes
  • Suitable for: ICC/IF, IHC-Fr, IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

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Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR19012]
    See all TBR2 / Eomes primary antibodies
  • Description

    Rabbit monoclonal [EPR19012] to TBR2 / Eomes
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC/IF: Mouse primary neuron cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19012
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Lineage specification
    • Trophectoderm
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-TBR2 / Eomes antibody [EPR19012] (ab222114)
    • Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TBR2 / Eomes protein (ab152851)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/200.
IHC-Fr (3)
1/1000.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/200.
IHC-Fr
1/1000.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
  • Tissue specificity

    Expressed in CD8+ T-cells.
  • Involvement in disease

    Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Developmental stage

    Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O95936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13813 Mouse
    • SwissProt: O54839 Mouse
    • Unigene: 200692 Mouse
    • Alternative names

      • eomes antibody
      • EOMES_HUMAN antibody
      • Eomesodermin antibody
      • Eomesodermin homolog antibody
      • t box brain 2 antibody
      • T-box brain protein 2 antibody
      • T-brain-2 antibody
      • Tbr 2 antibody
      • TBR-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on E14.5 mouse cortex is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on adult mouse hippocampus is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Negative control tissue; very weak staining is due to autoflorescence of the kidney section.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
      Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (26)

    Publishing research using ab183991? Please let us know so that we can cite the reference in this datasheet.

    ab183991 has been referenced in 26 publications.

    • Ezan J  et al. Early loss of Scribble affects cortical development, interhemispheric connectivity and psychomotor activity. Sci Rep 11:9106 (2021). PubMed: 33907211
    • Harlan De Crescenzo A  et al. Deficient or Excess Folic Acid Supply During Pregnancy Alter Cortical Neurodevelopment in Mouse Offspring. Cereb Cortex 31:635-649 (2021). PubMed: 32995858
    • Morandell J  et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nat Commun 12:3058 (2021). PubMed: 34031387
    • Ichinose M  et al. The BMP antagonist gremlin 1 contributes to the development of cortical excitatory neurons, motor balance and fear responses. Development 148:N/A (2021). PubMed: 34184027
    • Johnston S  et al. AAV ablates neurogenesis in the adult murine hippocampus. Elife 10:N/A (2021). PubMed: 34259630
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Adult brain hippocampus)
    Specification
    Adult brain hippocampus
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Aixa Morales

    Verified customer

    Submitted Aug 11 2020

    IHC - Wholemount abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (Wholemount mouse early embryo)
    Specification
    Wholemount mouse early embryo
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Nicolas Porchet

    Verified customer

    Submitted Oct 27 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, postnatal day 6)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, postnatal day 6
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    Submitted Aug 23 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Embryonic mouse brain coronal tissue section E.17)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer
    Permeabilization
    Yes - Triton-X-100
    Specification
    Embryonic mouse brain coronal tissue section E.17
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Carnoy's solution
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 18 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (embryonic mouse brain coronal sections E.16)
    Specification
    embryonic mouse brain coronal sections E.16
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 14 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain / DG)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Brain / DG
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Wei Mo

    Verified customer

    Submitted Dec 22 2016

    Immunohistochemistry free floating abreview for Anti-TBR2 / Eomes antibody [EPR19012]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Adult dentate gyrus)
    Specification
    Adult dentate gyrus
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 09 2016

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