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RecombinantRabMAb

Recombinant Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
  • Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19012] to TBR2 / Eomes - BSA and Azide free
  • Suitable for: ICC/IF, IHC-Fr, IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

Overview

  • Product name

    Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free
    See all TBR2 / Eomes primary antibodies

  • Description

    Rabbit monoclonal [EPR19012] to TBR2 / Eomes - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse hippocampus and E14 cortex tissues; IHC-Fr: Mouse E14.5 cortex and hippocampus tissues; ICC/IF: Mouse primary neuron cells.

  • General notes

    ab222226 is the carrier-free version of ab183991.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

  • Tissue specificity

    Expressed in CD8+ T-cells.

  • Involvement in disease

    Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.

  • Sequence similarities

    Contains 1 T-box DNA-binding domain.

  • Developmental stage

    Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • eomes antibody
      • EOMES_HUMAN antibody
      • Eomesodermin antibody
      • Eomesodermin homolog antibody
      • t box brain 2 antibody
      • T-box brain protein 2 antibody
      • T-brain-2 antibody
      • Tbr 2 antibody
      • TBR-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on intermediate neuronal progenitors of mouse hippocampus is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunocytochemistry/ Immunofluorescence - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TBR2 / Eomes with ab183991 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on E14.5 mouse cortex is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of paraffin-embedded mouse E14 cortex tissue labeling TBR2 / Eomes with ab183991 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on the cortex of mouse E14 is observed [PMID: 22553033]. 

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Specific staining on adult mouse hippocampus is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Immunohistochemistry (Frozen sections) - Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen adult mouse kidney tissue labeling TBR2 / Eomes with ab183991 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Negative control tissue; very weak staining is due to autoflorescence of the kidney section.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183991).

    • Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)
      Anti-TBR2 / Eomes antibody [EPR19012] - BSA and Azide free (ab222226)

    References (0)

    Publishing research using ab222226? Please let us know so that we can cite the reference in this datasheet.

    ab222226 has not yet been referenced specifically in any publications.

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