Anti-Tbx6 antibody (ab38883)
Key features and details
- Rabbit polyclonal to Tbx6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Tbx6 antibody
See all Tbx6 primary antibodies -
Description
Rabbit polyclonal to Tbx6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse Tbx6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab26243) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation. -
Tissue specificity
Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Developmental stage
Expressed during gastrulation and during a second phase in some adult tissues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6911 Human
- Entrez Gene: 21389 Mouse
- Omim: 602427 Human
- SwissProt: O95947 Human
- SwissProt: P70327 Mouse
- Unigene: 198301 Human
- Unigene: 727 Mouse
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Alternative names
- DFNB 67 antibody
- DFNB67 antibody
- SCDO5 antibody
see all
Images
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Western blot - Anti-Tbx6 antibody (ab38883)All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab38883 has been referenced in 4 publications.
- Bredenkamp N et al. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports 13:1083-1098 (2019). PubMed: 31708477
- Liu C et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). PubMed: 30008328
- Chal J et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). PubMed: 27583644
- Gouti M et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . PubMed: 25157815