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Anti-TDP43 antibody [2E2-D3] (ab57105)

Reviews (1)Q&A (1)References (24)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Sandwich ELISA - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Flow Cytometry - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
  • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)

Key features and details

  • Mouse monoclonal [2E2-D3] to TDP43
  • Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-TDP43 antibody [2E2-D3]
    See all TDP43 primary antibodies

  • Description

    Mouse monoclonal [2E2-D3] to TDP43

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant full length protein (GST-tag) corresponding to Human TDP43 aa 1-261. The molecular weight of GST-tag is 26kDa.
    Sequence:

    MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQC MRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAV QKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFV RFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTE DMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLII KGISVHISNA


    Database link: Q13148
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    This product was changed from ascites to tissue culture supernatant on 5/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab57105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (1)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.

Target

  • Function

    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

  • Tissue specificity

    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

  • Involvement in disease

    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Domain

    The RRM domains can bind to both DNA and RNA.

  • Post-translational
    modifications

    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

  • Cellular localization

    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALS10 antibody
      • OTTHUMP00000002171 antibody
      • OTTHUMP00000002172 antibody
      • OTTHUMP00000002173 antibody
      • TADBP_HUMAN antibody
      • TAR DNA binding protein 43 antibody
      • TAR DNA binding protein antibody
      • TAR DNA-binding protein 43 antibody
      • TARDBP antibody
      • TDP 43 antibody
      • TDP-43 antibody
      • TDP43 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [2E2-D3] (ab57105)

      ab57105 staining TDP43 in human leiomyosarcoma tissue. Antibody concentration 3µg/ml.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)

      TDP43 antibody (ab57105) at 1ug/lane + A-431 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)

      ab57105 staining TDP43 in HeLa cells. Antibody concentration 10µg/ml.

      This image was generated using the ascites version of the product.

       

       

    • Sandwich ELISA - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Sandwich ELISA - Anti-TDP43 antibody [2E2-D3] (ab57105)

      Detection limit for recombinant GST tagged TDP43 is 0.3 ng/ml as a capture antibody.

      This image was generated using the ascites version of the product.

       

    • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)

      ab57105 staining TDP43 overexpressed in the HEK293 cell line (cotransfected with TDP43 validated Chimera siRNA) (lane 1) or non-tranfected control (lane 2).

      GAPDH used as a specificity and loading control.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Flow Cytometry - Anti-TDP43 antibody [2E2-D3] (ab57105)

      Overlay histogram showing HeLa cells stained with ab57105 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57105, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
      This image was generated using the ascites version of the product.

    • Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Western blot - Anti-TDP43 antibody [2E2-D3] (ab57105)
      All lanes : Anti-TDP43 antibody [2E2-D3] (ab57105) at 5 µg/ml

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TDP43, cell lysate
      Lane 2 : Untransfected HEK-293T cell lysate

      Developed using the ECL technique.


      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)
      Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [2E2-D3] (ab57105)

      TARDBP antibody (ab57105) used in immunofluorescence at 10ug/ml on HeLa cells.

      This image was generated using the ascites version of the product.

    References (24)

    Publishing research using ab57105? Please let us know so that we can cite the reference in this datasheet.

    ab57105 has been referenced in 24 publications.

    • Kow RL  et al. Loss of aly/ALYREF suppresses toxicity in both tau and TDP-43 models of neurodegeneration. Geroscience 44:747-761 (2022). PubMed: 35122183
    • Latimer CS  et al. TDP-43 promotes tau accumulation and selective neurotoxicity in bigenic Caenorhabditis elegans. Dis Model Mech 15:N/A (2022). PubMed: 35178571
    • Serafín V  et al. Disposable immunoplatforms for the simultaneous determination of biomarkers for neurodegenerative disorders using poly(amidoamine) dendrimer/gold nanoparticle nanocomposite. Anal Bioanal Chem 413:799-811 (2021). PubMed: 32474723
    • Saxton AD & Kraemer BC Human Ubiquilin 2 and TDP-43 copathology drives neurodegeneration in transgenic Caenorhabditis elegans. G3 (Bethesda) 11:N/A (2021). PubMed: 33963840
    • Nielsen AK  et al. TDP-43-specific Autoantibody Decline in Patients With Amyotrophic Lateral Sclerosis. Neurol Neuroimmunol Neuroinflamm 8:N/A (2021). PubMed: 33361387
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-TDP43 antibody [2E2-D3]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (myoblase and C2C12)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    3 µg
    Specification
    myoblase and C2C12
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. wei xia

    Verified customer

    Submitted Feb 25 2019

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