Recombinant Anti-TDP43 antibody [DB9] (ab254166)
![Western blot - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367752-antitpd43-antibody-db9-western-blot.jpg "Western blot - Anti-TDP43 antibody [DB9] (ab254166)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [DB9] to TDP43
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TDP43 antibody [DB9]
See all TDP43 primary antibodies -
Description
Mouse monoclonal [DB9] to TDP43 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Common marmoset
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Immunogen
Recombinant fragment within Human TDP43 aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13148 -
Positive control
- WB: HeLa, Jurkat, K562, HEK-293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum and colon tissue; mouse cerebrum tissue; rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
DB9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.467 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
|
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| IHC-P | (4) |
Use a concentration of 4.67 - 23.35 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Human: 4.67µg/ml Mouse and rat: 23.35µg/ml |
| ELISA |
Use at an assay dependent concentration.
1000-10000 ng/mL |
| Notes |
|---|
|
WB
Use a concentration of 0.467 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
|
IHC-P
Use a concentration of 4.67 - 23.35 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Human: 4.67µg/ml Mouse and rat: 23.35µg/ml |
|
ELISA
Use at an assay dependent concentration. 1000-10000 ng/mL |
Target
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Function
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. -
Tissue specificity
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. -
Involvement in disease
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The RRM domains can bind to both DNA and RNA. -
Post-translational
modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
Cellular localization
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. - Information by UniProt
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Database links
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Entrez Gene: 298648 Rat
- Omim: 605078 Human
- SwissProt: Q13148 Human
- SwissProt: Q921F2 Mouse
- Unigene: 300624 Human
- Unigene: 635053 Human
see all -
Alternative names
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
Images
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Western blot - Anti-TDP43 antibody [DB9] (ab254166)All lanes : Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 4 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-4: 103 seconds;
Lanes 5-8: 48 seconds. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367756-antitpd43-antibody-db9-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TDP43 with ab254166 at 4.67µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367755-antitpd43-antibody-db9-immunohistochemistry-cerebrum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367754-antitpd43-antibody-db9-immunohistochemistry-cerebrum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TDP43 with ab254166 at 23.25µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367753-antitpd43-antibody-db9-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (ab254166)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TDP43 with ab254166 at 4.67µg/ml followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![ELISA - Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-367757-antitpd43-antibody-db9-elisa.jpg)
ELISA - Anti-TDP43 antibody [DB9] (ab254166)TDP43 = 5000 ng/mL.
ab254166 used at 0 - 10,000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）secondary antibody was used at 1000ng/ml
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![Anti-TDP43 antibody [DB9] (ab254166)](https://www.abcam.com/ps/products/254/ab254166/Images/ab254166-7-benefits-of-recombinant-antibodies.png)
Anti-TDP43 antibody [DB9] (ab254166)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab254166 has not yet been referenced specifically in any publications.