Recombinant Anti-TdT antibody [EPR9732] (ab155082)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)](https://www.abcam.com/ps/products/155/ab155082/Images/ab155082-161964-anti-tdt-antibody-epr9732-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9732] to TdT
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TdT antibody [EPR9732]
See all TdT primary antibodies -
Description
Rabbit monoclonal [EPR9732] to TdT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TdT.
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Positive control
- Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9732 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. -
Sequence similarities
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1791 Human
- Omim: 187410 Human
- SwissProt: P04053 Human
- Unigene: 534206 Human
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Alternative names
- Deoxynucleotidyltransferase terminal antibody
- DNA nucleotidylexotransferase antibody
- DNTT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)](https://www.abcam.com/ps/products/155/ab155082/Images/ab155082-161963-anti-tdt-antibody-epr9732-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-TdT antibody [EPR9732] (ab155082)](https://www.abcam.com/ps/products/155/ab155082/Images/ab155082-161962-anti-tdt-antibody-epr9732-western-blot.jpg)
Western blot - Anti-TdT antibody [EPR9732] (ab155082)All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution
Lane 1 : Human fetal thymus cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa -
![Anti-TdT antibody [EPR9732] (ab155082)](https://www.abcam.com/ps/products/155/ab155082/Images/ab155082-4-benefits-of-recombinant-antibodies.png)
Anti-TdT antibody [EPR9732] (ab155082)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155082 has been referenced in 1 publication.
- Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). PubMed: 25775545