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RecombinantRabMAb

Recombinant Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
  • Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
  • Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-TEM8/ATR antibody [EPNCI-R173-37]
    See all TEM8/ATR primary antibodies

  • Description

    Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mammary fat pad of TEM8/ATR wildtype (WT) athymic nude mice transplanted with HT29 human colon tumor. ICC/IF: SW480 and LoVo cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab241067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

(Antigen Retrieval with proteinase K).
ICC/IF
1/100.
Notes
IHC-P
1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

(Antigen Retrieval with proteinase K).
ICC/IF
1/100.
Application notes
Is unsuitable for Flow Cyt or WB.

Target

  • Function

    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.

  • Tissue specificity

    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.

  • Involvement in disease

    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • Sequence similarities

    Belongs to the ATR family.
    Contains 1 VWFA domain.

  • Domain

    Binding to PA occurs through the VWA domain.

  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt

  • Database links

    • Alternative names

      • Anthrax toxin receptor 1 antibody
      • ANTR1_HUMAN antibody
      • Antxr1 antibody
      • ATR antibody
      • Tumor endothelial marker 8 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
      Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

      Immunofluorescent analysis of 100% Methanol fixed LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in LoVo cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      PFA fixation is not recommended due to weak staining on LoVo cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

      Immunohistochemical analysis of 10% neutral-buffered formalin Mammary fat pad of TEM8/ATR wildtype (WT) or knockout (KO) athymic nude mice transplanted with HT29 human colon tumor tissue labeling TEM8/ATR with ab241067 at 1/60 dilution, followed by a Biotin anti-rabbit IgG secondsry antibody. Positive staining in stromal cells of transplanted HT29 human colon tumor growing in TEM8/ATR wildtype (WT) athymic nude mice is observed.

      Antigen retrieval: Retrieved with proteinase K.

      This image was kindly provided by our collaborator Dr. Brad St. Croix, National Cancer Institute (NCI), NIH, and published in J Clin Invest. 128: 2927–2943 (PMID:29863500).

    • Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
      Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

      Immunofluorescent analysis of 100% Methanol fixed SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in SW480 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      PFA fixation is not recommended due to negative staining on SW480 cells.

       

       

    • Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
      Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab241067? Please let us know so that we can cite the reference in this datasheet.

    ab241067 has been referenced in 1 publication.

    • Szot C  et al. Tumor stroma-targeted antibody-drug conjugate triggers localized anticancer drug release. J Clin Invest 128:2927-2943 (2018). PubMed: 29863500

    Customer reviews and Q&As

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