Anti-TEX12 antibody (ab122455)
Key features and details
- Rabbit polyclonal to TEX12
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEX12 antibody -
Description
Rabbit polyclonal to TEX12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MANHLVKPDN RNCKRPRELE SPVPDSPQLS SLGKSDSSFS EISGLFYKDE ALEKDLNDVS, corresponding to amino acids 2-61 of Human TEX12.
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Positive control
- Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
| Notes |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Tissue specificity
Testis specific. - Information by UniProt
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Database links
- Entrez Gene: 56158 Human
- Omim: 605791 Human
- SwissProt: Q9BXU0 Human
- Unigene: 524039 Human
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Alternative names
- Testis expressed gene 12 antibody
- Testis expressed sequence 12 protein variant 3 antibody
- Testis-expressed sequence 12 protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TEX12 antibody (ab122455)Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-TEX12 antibody (ab122455)Lanes 2-3 : Anti-TEX12 antibody (ab122455)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEX12 antibody (ab122455)ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122455 has been referenced in 1 publication.
- Sandhu S et al. Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12. Commun Biol 4:1371 (2021). PubMed: 34880391