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RecombinantRabMAb

Recombinant Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
  • Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15346] to TGF alpha - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TGF alpha antibody [EPR15346] - BSA and Azide free
    See all TGF alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR15346] to TGF alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal skin, fetal liver and breast cancer lysates; HepG2 and Ramos whole cell lysates. IHC-P: Human liver and hepatocellular carcinoma tissues. ICC/IF: HepG2 cells. IP: HepG2 and Ramos whole cell lysates.

  • General notes

    ab224266 is the carrier-free version of ab208156.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.

  • Sequence similarities

    Contains 1 EGF-like domain.

  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • EGF like TGF antibody
      • EGF-like TGF antibody
      • ETGF antibody
      • TFGA antibody
      • TGF A antibody
      • TGF type 1 antibody
      • TGF-alpha antibody
      • Tgfa antibody
      • TGFA_HUMAN antibody
      • Transforming growth factor alpha antibody
      • Transforming growth factor alpha precursor antibody
      • Wa1 antibody
      • Waved 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling TGF alpha with ab208156 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on the cancer cells of Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling TGF alpha with ab208156 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody  -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab208156 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

    • Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunocytochemistry/ Immunofluorescence - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells, treated with Brefeldin A (10 µM, 18 hours), labeling TGF alpha with ab208156 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cells, treated with Brefeldin A (10 µM, 18 hours). The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab208156 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

    • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      TGF alpha was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab208156 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208156 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HepG2 whole cell lysate 10µg (Input).

      Lane 2: ab208156 IP in HepG2 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab208156 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

    • Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunoprecipitation - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      TGF alpha was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab208156 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208156 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Ramos whole cell lysate, 10µg (Input).

      Lane 2: ab208156 IP in Ramos whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab208156 in Ramos whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208156).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

      This IHC data was generated using the same anti-TGF alpha antibody clone [EPR15346] in a different buffer formulation (cat# ab208156).

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TGF alpha with ab208156 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human liver is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)
      Anti-TGF alpha antibody [EPR15346] - BSA and Azide free (ab224266)

    References (0)

    Publishing research using ab224266? Please let us know so that we can cite the reference in this datasheet.

    ab224266 has not yet been referenced specifically in any publications.

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