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    products/primary-antibodies/tgf-beta-1-antibody-epr18163-ab179695.pdf

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Cardiovascular Angiogenesis Growth Factors TGF
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TGF beta 1 antibody [EPR18163] (ab179695)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (44)

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Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
  • Anti-TGF beta 1 antibody [EPR18163] (ab179695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18163] to TGF beta 1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Human TGFB1 (TGF beta 1) knockout HeLa cell line (ab255439)

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Overview

  • Product name

    Anti-TGF beta 1 antibody [EPR18163]
    See all TGF beta 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18163] to TGF beta 1
  • Host species

    Rabbit
  • Specificity

    ab179695 recognizes the mature and cleaved forms of TGF-beta 1.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: L-929, RAW 264.7, HeLa, Wild-type A549, A549, SH-SY5Y and K562 whole cell lysates; Mouse serum; Mouse spleen lysate; Rat kidney and spleen lysates; Rat serum; Human kidney and spleen lysates; Recombinant protein fragment human TGF beta 1.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18163
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Secreted
    • Cancer
    • Growth factors
    • TGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-TGF beta 1 antibody [EPR18163] - BSA and Azide free (ab233730)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human TGFB1 (TGF beta 1) knockout HeLa cell line (ab255439)
    • Human TGFB1 (TGF beta 1) knockout A549 cell line (ab269509)
  • KO cell lysates

    • Human TGFB1 (TGF beta 1) knockout HeLa cell lysate (ab263799)
    • Human TGFB1 (TGF beta 1) knockout A549 cell lysate (ab269671)
  • Recombinant Protein

    • Recombinant human TGF beta 1 protein (Active) (ab50036)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000. Detects a band of approximately 50, 12.5 kDa (predicted molecular weight: 44 kDa).
Notes
WB
1/1000. Detects a band of approximately 50, 12.5 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P01137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7040 Human
    • Entrez Gene: 21803 Mouse
    • Entrez Gene: 59086 Rat
    • Omim: 190180 Human
    • SwissProt: P01137 Human
    • SwissProt: P04202 Mouse
    • SwissProt: P17246 Rat
    • Unigene: 645227 Human
    • Unigene: 248380 Mouse
    • Unigene: 40136 Rat
    see all
  • Alternative names

    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

Images

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TGFB1 knockout A549 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab179695 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab179695 was shown to react with TGF beta  in wild-type A549 cells in Western blot with loss of signal observed in TGFB1 knockout cell line ab269509 (TGFB1 knockout cell lysate ab269671). Wild-type A549 and TGFB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab179695 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : TGF beta 1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab179695 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab179695 was shown to react with TGF beta 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255439 (knockout cell lysate ab263799) was used. Wild-type and TGF beta 1 knockout samples were subjected to SDS-PAGE. ab179695 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution

    Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2-4:30 seconds.

     

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Lane 1 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
    Lanes 2-5 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Rat serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2/3/5: 3minutes; Lane 4: 30 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).

  • Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution

    Lane 1 : Recombinant protein fragment human TGF beta 1
    Lane 2 : Recombinant protein fragment human TGF beta 2
    Lane 3 : Recombinant protein fragment human TGF beta 3

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2/3: 3 minutes.

    Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house. 

  • Anti-TGF beta 1 antibody [EPR18163] (ab179695)
    Anti-TGF beta 1 antibody [EPR18163] (ab179695)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (44)

Publishing research using ab179695? Please let us know so that we can cite the reference in this datasheet.

ab179695 has been referenced in 44 publications.

  • Wang Q  et al. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J Basic Med Sci 21:81-92 (2021). PubMed: 32343942
  • Liang Y  et al. Silenced SOX2-OT alleviates ventricular arrhythmia associated with heart failure by inhibiting NLRP3 expression via regulating miR-2355-3p. Immun Inflamm Dis 9:255-264 (2021). PubMed: 33270361
  • Wei X  et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
  • Liu X  et al. Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1. Cancer Cell Int 21:43 (2021). PubMed: 33430878
  • Miao J  et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-TGF beta 1 antibody [EPR18163]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (colon)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
15 µg
Specification
colon
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 01 2021

Western blot abreview for Anti-TGF beta 1 antibody [EPR18163]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Recombinant protein (Total protein extracted from transiently transform)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
7000 µg
Specification
Total protein extracted from transiently transform
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 26 2020

Western blot abreview for Anti-TGF beta 1 antibody [EPR18163]

Good
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 06 2018

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