Anti-TGF beta 1 antibody [TB21] (ab190503)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [TB21] (ab190503)](https://www.abcam.com/ps/products/190/ab190503/Images/ab190503-225591-anti-tgf-beta-1-antibody-tb21-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [TB21] (ab190503)")
Key features and details
- Mouse monoclonal [TB21] to TGF beta 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TGF beta 1 antibody [TB21]
See all TGF beta 1 primary antibodies -
Description
Mouse monoclonal [TB21] to TGF beta 1 -
Host species
Mouse -
Specificity
ab190503 recognizes both human platelet-derived and recombinant TGF beta1 in enzyme-linked immunosorbent assay (ELISA). Mouse anti-Human TGF beta antibody, clone TB21 demonstrates neutralising activity against TGF beta1 in cell proliferation assays. Mouse anti Human TGF beta antibody, clone TB21 has been demonstrated to react with dimeric (25kD) or monomeric (12.5kD) molecules of natural TGF beta1 under non-reducing and reducing conditions respectively. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Guinea pig, Dog, Pig
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Immunogen
Full length native protein (purified) corresponding to Human TGF beta 1 aa 250 to the C-terminus. purified from platelets.
Database link: P01137 -
Positive control
- Human breast carcinoma and brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TB21 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
ab190503 has been used successfully on FFPE tissues without pretreatment. Citrate pH6.0, EDTA pH8.0 or pepsin mediated antigen retrieval have also been used. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. ab190503 has been used successfully on FFPE tissues without pretreatment. Citrate pH6.0, EDTA pH8.0 or pepsin mediated antigen retrieval have also been used. |
Target
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Function
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
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Alternative names
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [TB21] (ab190503)
Immunohistochemical analysis of Human brain tissue labeling TGF beta with ab190503.
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab190503 has been referenced in 30 publications.
- Yu L et al. Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography. Asian J Androl 24:207-212 (2022). PubMed: 34494557
- Xiong G et al. Long non-coding RNA MEG3 regulates the progress of osteoarthritis by regulating the miR-34a/Klotho axis. Ann Transl Med 10:454 (2022). PubMed: 35571440
- Souza-Neto FV et al. Mitochondrial Oxidative Stress Promotes Cardiac Remodeling in Myocardial Infarction through the Activation of Endoplasmic Reticulum Stress. Antioxidants (Basel) 11:N/A (2022). PubMed: 35883722
- Leung D et al. Platinum-resistance in epithelial ovarian cancer: an interplay of epithelial-mesenchymal transition interlinked with reprogrammed metabolism. J Transl Med 20:556 (2022). PubMed: 36463238
- Kim IY et al. Role of Akt1 in renal fibrosis and tubular dedifferentiation during the progression of acute kidney injury to chronic kidney disease. Korean J Intern Med 36:962-974 (2021). PubMed: 33322851