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    products/primary-antibodies/tgfbi-antibody-epr12078b-ab170874.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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RecombinantRabMAb

Recombinant Anti-TGFBI antibody [EPR12078(B)] (ab170874)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (21)

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Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Anti-TGFBI antibody [EPR12078(B)] (ab170874)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12078(B)] to TGFBI
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human TGFBI protein (ab51281)

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Overview

  • Product name

    Anti-TGFBI antibody [EPR12078(B)]
    See all TGFBI primary antibodies
  • Description

    Rabbit monoclonal [EPR12078(B)] to TGFBI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TGFBI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control

    • Human fetal kidney and Human fetal liver lysates, Human colon tissue and Human uterus tissue, Y79 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12078(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Neuroscience
    • Sensory System
    • Visual system
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-TGFBI antibody [EPR12078(B)] - BSA and Azide free (ab240162)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TGFBI protein (ab51281)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
ICC/IF
1/50 - 1/100.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
  • Tissue specificity

    Highly expressed in the corneal epithelium.
  • Involvement in disease

    Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
    Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
    Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
    Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
    Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
    Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
    Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
  • Sequence similarities

    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
  • Target information above from: UniProt accession Q15582 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7045 Human
    • Entrez Gene: 21810 Mouse
    • Entrez Gene: 116487 Rat
    • Omim: 601692 Human
    • SwissProt: Q15582 Human
    • SwissProt: P82198 Mouse
    • Unigene: 369397 Human
    • Unigene: 14455 Mouse
    • Alternative names

      • RGD containing collagen associated protein antibody
      • AI181842 antibody
      • AI747162 antibody
      • Beta ig antibody
      • Beta ig h3 antibody
      • Beta ig-h3 antibody
      • BGH3_HUMAN antibody
      • Big h3 antibody
      • BIGH3 antibody
      • CDB1 antibody
      • CDG2 antibody
      • CDGG1 antibody
      • CSD antibody
      • CSD1 antibody
      • CSD2 antibody
      • CSD3 antibody
      • EBMD antibody
      • Kerato epithelin antibody
      • Kerato-epithelin antibody
      • LCD1 antibody
      • MGC150270 antibody
      • RGD CAP antibody
      • RGD-CAP antibody
      • RGD-containing collagen-associated protein antibody
      • TGFBI antibody
      • TGFBI transforming growth factor, beta induced, 68kDa antibody
      • Transforming growth factor beta induced protein ig h3 antibody
      • Transforming growth factor-beta-induced protein ig-h3 antibody
      see all

    Images

    • Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      All lanes : Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 75 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Anti-TGFBI antibody [EPR12078(B)] (ab170874)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (21)

    Publishing research using ab170874? Please let us know so that we can cite the reference in this datasheet.

    ab170874 has been referenced in 21 publications.

    • Song Z  et al. Metformin attenuates post-epidural fibrosis by inhibiting the TGF-ß1/Smad3 and HMGB1/TLR4 signaling pathways. J Cell Mol Med 25:3272-3283 (2021). PubMed: 33611840
    • Hu Y  et al. Angelica Dahurica Regulated the Polarization of Macrophages and Accelerated Wound Healing in Diabetes: A Network Pharmacology Study and In Vivo Experimental Validation. Front Pharmacol 12:678713 (2021). PubMed: 34234674
    • Wang L  et al. Torin 1 alleviates impairment of TFEB-mediated lysosomal biogenesis and autophagy in TGFBI (p.G623_H626del)-linked Thiel-Behnke corneal dystrophy. Autophagy N/A:1-18 (2021). PubMed: 34403298
    • Leng L  et al. Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Stem Cell Res Ther 11:415 (2020). PubMed: 32967725
    • Lokhnauth J  et al. Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis. J Exp Orthop 7:39 (2020). PubMed: 32476071
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human fetal membranes)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization
    Yes - 0.5% Tween
    Specification
    Human fetal membranes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Katharina Gegenschatz

    Verified customer

    Submitted Apr 20 2022

    Immunohistochemistry (Frozen sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Tonsillen-CA)
    Permeabilization
    Yes - Acetone
    Specification
    Tonsillen-CA
    Blocking step
    Serum as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 12 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse brain tumor)
    Permeabilization
    No
    Specification
    Mouse brain tumor
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 13 2020

    Western blot abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse brain tumor)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    5 µg
    Specification
    mouse brain tumor
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 23 2018

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