Recombinant Anti-Thiophosphate ester antibody [51-8] (ab133473)
![Immunoprecipitation - Anti-Thiophosphate ester antibody [51-8] (ab133473)](https://www.abcam.com/ps/products/133/ab133473/Images/ab133473-147115-anti-thiophosphate-ester-antibody-51-8-immunoprecipitation.jpg "Immunoprecipitation - Anti-Thiophosphate ester antibody [51-8] (ab133473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [51-8] to Thiophosphate ester
- Suitable for: IP
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-Thiophosphate ester antibody [51-8]
See all Thiophosphate ester primary antibodies -
Description
Rabbit monoclonal [51-8] to Thiophosphate ester -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.
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Positive control
- HeLa cell lysate
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General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.
This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
See specific Protocol at the bottom of this page for details.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
See specific Protocol at the bottom of this page for details. -
Clonality
Monoclonal -
Clone number
51-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | (1) |
1/5000.
Recommended: 0.5-1 ug of antibody per 1ug of kinase substrate |
| Notes |
|---|
|
IP
1/5000. Recommended: 0.5-1 ug of antibody per 1ug of kinase substrate |
Target
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Relevance
Thiophosphate ester is used as fungicide.
Images
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Immunoprecipitation - Anti-Thiophosphate ester antibody [51-8] (ab133473)ab133473, at 1:5000, immunoblotting immunoprecipitated samples pre-resolved by SDS-PAGE. Myelin basic protein (MBP) was thiophosphorylated by AS GSK3 beta (GSK3 beta also catalyzes an auto thiophosporylation reaction). Samples were treated with or without p-nitrobenzyl mesylate (PNBM) for 1 hour at room temperature. PNBM was removed by size exclusion and 1mg/ml of HeLa cell lysate was added to samples.
ab133473 was pre-incubated with protein G beads and was added to sample for an immunoprecipitation at 4°C overnight:
(1) Thiophosphorylated MBP
(2) PNBM treated thiophosphorylated MBP sample
(3) HeLa cell lysate
(4) HeLa cell lysate with PNBM treated thiophosphorylated MBP sample
(5) Control -
![Anti-Thiophosphate ester antibody [51-8] (ab133473)](https://www.abcam.com/ps/products/133/ab133473/Images/ab133473-2-benefits-of-recombinant-antibodies.png)
Anti-Thiophosphate ester antibody [51-8] (ab133473)
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab133473 has been referenced in 11 publications.
- Zhang H et al. A subcellular map of the human kinome. Elife 10:N/A (2021). PubMed: 33988507
- Yao W et al. Atg11 is required for initiation of glucose starvation-induced autophagy. Autophagy 16:2206-2218 (2020). PubMed: 31971848
- Dibus M et al. A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18. Int J Mol Sci 21:N/A (2020). PubMed: 33092266
- Michowski W et al. Cdk1 Controls Global Epigenetic Landscape in Embryonic Stem Cells. Mol Cell 78:459-476.e13 (2020). PubMed: 32240602
- Shehata SN et al. Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. Cell Signal 59:53-61 (2019). PubMed: 30880224