Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Thrombin antibody (ab48626)

Submit a review Q&A (2)

Key features and details

  • Rabbit polyclonal to Thrombin
  • Suitable for: ELISA, EIA, IP, IHC-P, ICC/IF, RIA, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Prothrombin antibody [EPR20131] (ab208590)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Thrombin antibody
    See all Thrombin primary antibodies

  • Description

    Rabbit polyclonal to Thrombin

  • Host species

    Rabbit

  • Specificity

    The antibody will recognize prothrombin and thrombin

  • Tested applications

    Suitable for: ELISA, EIA, IP, IHC-P, ICC/IF, RIA, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein: Human thrombin purified from human plasma

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab48626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Function

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Involvement in disease

    Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC 3.4.21.5 antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • Prothrombin B chain antibody
      • PT antibody
      • RPRGL2 antibody
      • Serine protease antibody
      • THPH1 antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all

    References (0)

    Publishing research using ab48626? Please let us know so that we can cite the reference in this datasheet.

    ab48626 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    LOT NUMBER 835349 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands SAMPLE Blood serum / plasma PRIMARY ANTIBODY Abcam:ab48626 DETECTION METHOD Biorad OPTI-4CN POSITIVE AND NEGATIVE CONTROLS USED Thrombin ANTIBODY STORAGE CONDITIONS -20oC until first use SAMPLE PREPARATION none AMOUNT OF PROTEIN LOADED 500 ng ELECTROPHORESIS/GEL CONDITIONS reducing 4-20% TRANSFER AND BLOCKING CONDITIONS Tris/glycine/ sds methanol blocking: NFDM SECONDARY ANTIBODY Biorad anti-rabbit HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No ADDITIONAL NOTES We believe that you have mislabeled this antibody. Rather than being an antibody to Thrombin, we think it is an antibody to Antithrombin. We have performed several other western blots with anti-antithrombin antibodies (obtained from abcam) and get a similar pattern as with this antibody. With this antibody (ab48626), there should be a prominent band at 30kDa. Instead there is a prominent band at around 55-58kDa which is consistent with Antithrombin.

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab48626. I would also appreciate if you can confirm some further details:

    - Could you confirm when the product was ordered? This will help me trace the origin and internal lot details.
    - Reviewing the Swiss-prot entry, I would expect a band at ˜65-70 kDa to be observed, however you state that you would expect a band of 30 kDa. Why would you expect a band size of 30 kDa? The antibody should detect thrombin and prothrombin.
    - The antibody was generated against full length native protein so it is unlikely to recognize antihrombin.
    - Is it possible to obtain an image so I can understand the problem in more detail?
    - Was the sample prepared and resolved under reducing and denaturing conditions? The antibody has been characterized under such conditions.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Question

    DESCRIPTION OF THE PROBLEM we find no of multiple bands picking other than 37 KD , which does not pick with prothrombin antibodies SAMPLE purified thrombin PRIMARY ANTIBODY 1:2000 dilution with 1 hour incubation and 3 wash steps 5 mins DETECTION METHOD BCIP/NBT POSITIVE AND NEGATIVE CONTROLS USED market standard used gives same result ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION heating 3mins AMOUNT OF PROTEIN LOADED about 40-50 micrograms ELECTROPHORESIS/GEL CONDITIONS both reducing and non reducing conditions TRANSFER AND BLOCKING CONDITIONS overnight milk powder in TBST SECONDARY ANTIBODY anti rabbit IgG sigma

    Read More
    Answer

    Thank you for contacting me again. Detecting thrombin specifically is a very hard issue. As thrombin is part of pro thrombin and the proform undergoes activation during blood coagulation therefore it is difficult to differentiate both proteins. We have several products in our catalogue targeting thrombin, but they are all very likely to recognise pro thrombin as well. In order to obtain clearer thrombin bands, using protein degradation inhibitors are highly recommended (to avoid protein degradation products normally showing as lower target bands), as well as anti coagulants. Thrombin is a very special product, with different sites of glycosilation, producing bands at higher molecular weights. So in this particular case, sample preparation is crucial. However, I would be very happy to offer you a credit note for the price of this antibody, valid for a future purchase. I would really like to help you get the better results from the antibody, so please, let us know how you wish to proceed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com