Recombinant Anti-Thrombomodulin antibody [EPR18217] (ab187075)
![Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)](https://www.abcam.com/ps/products/187/ab187075/Images/ab187075-286144-anti-thrombomodulin-antibody-epr18217-western-blot.jpg "Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18217] to Thrombomodulin
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Thrombomodulin antibody [EPR18217]
See all Thrombomodulin primary antibodies -
Description
Rabbit monoclonal [EPR18217] to Thrombomodulin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEND.3 cell lysate; Rat lung and heart lysates; Mouse lung and placenta lysates. IHC-P: Mouse and rat lung tissues. Flow Cyt: bEND.3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 105, 75 kDa (predicted molecular weight: 62 kDa).
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
1/60.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 105, 75 kDa (predicted molecular weight: 62 kDa). |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt
1/60. |
Target
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Function
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
Tissue specificity
Endothelial cells are unique in synthesizing thrombomodulin. -
Involvement in disease
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
Post-translational
modificationsN-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21824 Mouse
- Entrez Gene: 83580 Rat
- SwissProt: P15306 Mouse
- Unigene: 24096 Mouse
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Alternative names
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
Images
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Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)All lanes : Anti-Thrombomodulin antibody [EPR18217] (ab187075) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 3 : Mouse placenta lysate
Lane 4 : Rat lung lysate
Lane 5 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 105,75 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 30 seconds; Lanes 4-5: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9619877).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)](https://www.abcam.com/ps/products/187/ab187075/Images/ab187075-286143-anti-thrombomodulin-antibody-epr18217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)](https://www.abcam.com/ps/products/187/ab187075/Images/ab187075-286142-anti-thrombomodulin-antibody-epr18217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat lung (PMID: 23946288). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217] (ab187075)](https://www.abcam.com/ps/products/187/ab187075/Images/ab187075-286141-anti-thrombomodulin-antibody-epr18217-flow-cytometry.jpg)
Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217] (ab187075)Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cell line labeling Thrombomodulin with ab187075 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Total viable cells were gated for the FC image.
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![Anti-Thrombomodulin antibody [EPR18217] (ab187075)](https://www.abcam.com/ps/products/187/ab187075/Images/ab187075-5-benefits-of-recombinant-antibodies.png)
Anti-Thrombomodulin antibody [EPR18217] (ab187075)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab187075 has been referenced in 2 publications.
- Cheng X et al. Identification of thrombomodulin as a dynamic monitoring biomarker for deep venous thrombosis evolution. Exp Ther Med 21:142 (2021). PubMed: 33456509
- Wei Y et al. Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors. Mol Biol Rep 47:6899-6918 (2020). PubMed: 32892299