Recombinant Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)
![Western blot - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-476139-anti-thymidine-phosphorylase-antibody-epr25629129-western-blot-lysate.jpg "Western blot - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25629-129] to Thymidine Phosphorylase
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Thymidine Phosphorylase antibody [EPR25629-129]
See all Thymidine Phosphorylase primary antibodies -
Description
Rabbit monoclonal [EPR25629-129] to Thymidine Phosphorylase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human tonsil, human ovary cancer, human colon and human liver, A431 lysates. IHC-P: Human tonsil and Human colon, Human ovarian cancer tissues. ICC/IF: A431, THP-1 cells. Flow Cyt: A431 cell. IP: A431 cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25629-129 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| WB |
1/1000. Predicted molecular weight: 50 kDa.
|
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| ICC/IF |
1/50.
|
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| IP |
1/30.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Predicted molecular weight: 50 kDa. |
|
ICC/IF
1/50. |
|
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
1/30. |
Target
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Function
May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.
Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. -
Pathway
Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2. -
Involvement in disease
Mitochondrial DNA depletion syndrome 1 (MTDPS1) [MIM:603041]: A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, cachexia, peripheral neuropathy, and myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. - Information by UniProt
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Database links
- Entrez Gene: 1890 Human
- Omim: 131222 Human
- SwissProt: P19971 Human
- Unigene: 180903 Human
- Unigene: 730607 Human
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Alternative names
- ECGF 1 antibody
- ECGF antibody
- ECGF1 antibody
see all
Images
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Western blot - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)All lanes : Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : human ovary cancer tissue lysate
Lane 3 : human ovary tissue lysate
Lane 4 : human colon tissue lysate
Lane 5 : human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP)(ab131366))
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: human ovary (PMID:2328255).
The bands nearby 25 kDa could be non-specific bands.
Exposure time: Lane 1-3: 3.25 seconds Lane 4-5: 37 seconds
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![Western blot - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-476140-anti-thymidine-phosphorylase-antibody-epr25629129-western-blot-carcinoma.jpg)
Western blot - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861) at 1/1000 dilution + A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 137 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-1-anti-thymidine-phosphorylase-antibody-epr25629129-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Thymidine phosphorylase with ab284861 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab284861 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-2-anti-thymidine-phosphorylase-antibody-epr25629129-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Thymidine phosphorylase with ab284861 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the interstitial cells of human colon (PMID:16361565). The section was incubated with ab284861 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-3-anti-thymidine-phosphorylase-antibody-epr25629129-immunohistochemistry-human-ovarian-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Thymidine phosphorylase with ab284861 at 1/5000 (0.103 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human ovarian cancer. The section was incubated with ab284861 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-4-anti-thymidine-phosphorylase-antibody-epr25629129-immunocytochemistry-a431.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling Thymidine phosphorylase with ab284861 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in A431 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-5-anti-thymidine-phosphorylase-antibody-epr25629129-immunocytochemistry-thp1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling Thymidine phosphorylase with ab284861 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
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![Flow Cytometry (Intracellular) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-6-anti-thymidine-phosphorylase-antibody-epr25629129-flow-cytometry-a431.jpg)
Flow Cytometry (Intracellular) - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cells labelling Thymidine phosphorylase with ab284861 at 1/500 dilution (0.1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730)/(Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody)/(Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)](https://www.abcam.com/ps/products/284/ab284861/Images/ab284861-7-anti-thymidine-phosphorylase-antibody-epr25629129-immunoprecipitation-a431.jpg)
Immunoprecipitation - Anti-Thymidine Phosphorylase antibody [EPR25629-129] (ab284861)Thymidine phosphorylase was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 ug with ab284861 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab284861 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab284861 IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab284861 in A431 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab284861 has not yet been referenced specifically in any publications.