For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/tie1-phospho-y1007--tie2-phospho-y992-antibody-epr1053nb-ab151704.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
Share by email
RecombinantRabMAb

Recombinant Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-VCAM1 antibody [EPR5047] (ab134047)
Primary
Product image
Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)]
  • Description

    Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)
  • Host species

    Rabbit
  • Specificity

    ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blotmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC cell lysate treated with pervanadate. ICC/IF: HUVEC cells treated with pervanadate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1053(N)(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Developmental Biology
    • Organogenesis
    • Angiogenesis and vasculogenesis
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free (ab229195)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
ICC/IF
1/250 - 1/500.
Dot blot
1/1000.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
ICC/IF
1/250 - 1/500.
Dot blot
1/1000.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Cellular localization

    TIE1: Cell membrane. TIE2: Cell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted.
  • Database links

    • Entrez Gene: 7010 Human
    • Entrez Gene: 7075 Human
    • Omim: 600221 Human
    • Omim: 600222 Human
    • SwissProt: P35590 Human
    • SwissProt: Q02763 Human
    • Unigene: 78824 Human
    • Unigene: 89640 Human

    Images

    • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : HUVEC cell lysate treated with pervanadate
      Lane 2 : HUVEC cell lysate treated with pervanadate with TIE2 (phospho Y992) peptide
      Lane 3 : HUVEC cell lysate treated with pervanadate with TIE2 unmodified peptide
      Lane 4 : HUVEC cell lysate treated with pervanadate with TIE1 (phospho Y1007) peptide
      Lane 5 : HUVEC cell lysate treated with pervanadate with TIE1 unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 5 seconds


      Blocking buffer: 5% BSA/TBST
      Dilution buffer: 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody

    • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

      Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

      Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

    • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : Untreated HUVEC whole cell lysates
      Lane 2 : HUVEC treated with Pervanadate whole cell lysates
      Lane 3 : HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 10 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

    • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

      Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab151704? Please let us know so that we can cite the reference in this datasheet.

    ab151704 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab151704.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.