Recombinant Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
![Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)](https://www.abcam.com/ps/products/151/ab151704/Images/ab151704-255429-anti-tie2-tie1-phospho-y992-y1007-antibody-epr1053nb-western-blot.jpg "Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] -
Description
Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992) -
Host species
Rabbit -
Specificity
ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.
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Tested applications
Suitable for: WB, ICC/IF, Dot blotmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC cell lysate treated with pervanadate. ICC/IF: HUVEC cells treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1053(N)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
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| ICC/IF |
1/250 - 1/500.
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 125 kDa. |
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ICC/IF
1/250 - 1/500. |
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Dot blot
1/1000. |
Target
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Cellular localization
TIE1: Cell membrane. TIE2: Cell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted. -
Database links
- Entrez Gene: 7010 Human
- Entrez Gene: 7075 Human
- Omim: 600221 Human
- Omim: 600222 Human
- SwissProt: P35590 Human
- SwissProt: Q02763 Human
- Unigene: 78824 Human
- Unigene: 89640 Human
Images
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Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution
Lane 1 : HUVEC cell lysate treated with pervanadate
Lane 2 : HUVEC cell lysate treated with pervanadate with TIE2 (phospho Y992) peptide
Lane 3 : HUVEC cell lysate treated with pervanadate with TIE2 unmodified peptide
Lane 4 : HUVEC cell lysate treated with pervanadate with TIE1 (phospho Y1007) peptide
Lane 5 : HUVEC cell lysate treated with pervanadate with TIE1 unmodified peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution (HRP goat anti-rabbit IgG (H+L))
Predicted band size: 125 kDa
Observed band size: 125 kDa
Exposure time: 5 secondsBlocking buffer: 5% BSA/TBST
Dilution buffer: 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody -
![Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)](https://www.abcam.com/ps/products/151/ab151704/Images/ab151704-264532-anti-tie2-tie1-phospho-y992-y1007-antibody-epr1053nb-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).
Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining.
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![Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)](https://www.abcam.com/ps/products/151/ab151704/Images/ab151704-264534-anti-tie2-tie1-phospho-y992-y1007-antibody-epr1053nb-western-blot.jpg)
Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution
Lane 1 : Untreated HUVEC whole cell lysates
Lane 2 : HUVEC treated with Pervanadate whole cell lysates
Lane 3 : HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
Exposure time: 10 secondsBlocking/Diluting buffer 5% NFDM/TBST
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![Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)](https://www.abcam.com/ps/products/151/ab151704/Images/ab151704-253326-anti-tie2-tie1-phospho-y992-y1007-antibody-epr1053nb-dot-blot.jpg)
Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)](https://www.abcam.com/ps/products/151/ab151704/Images/ab151704-7-benefits-of-recombinant-antibodies.png)
Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151704 has been referenced in 2 publications.
- Koya Y et al. An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1. Sci Rep 11:14021 (2021). PubMed: 34234265
- Messerschmidt VL et al. Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules. Front Cardiovasc Med 8:707897 (2021). PubMed: 34651022